Anti-Mouse E-Selectin – Biotin

Anti-Mouse E-Selectin – Biotin

Product No.: E291

[product_table name="All Top" skus="E291"]

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Target
E-Selectin
Product Type
Polyclonal Antibody
Alternate Names
SELE, CD62E, Endothelial Leukocyte Adhesion Molecule (ELAM), ELAM1, ESEL, LECAM2
Applications
IHC FFPE
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WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Chicken
Immunogen
Purified Recombinant Mouse E-Selectin (Accession # Q00690)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse E-Selectin this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E211.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Chicken Anti-Mouse E-Selectin recognizes Mouse E-Selectin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
E-selectin, also known as CD62E is an inducible leukocyte adhesion glycoprotein specifically expressed by endothelial cells.1 E-selectin helps initiate recruitment of circulating leukocytes to cutaneous, bone and inflamed tissues.2 E-selectin is found in inflammatory skin lesions in psoriasis, contact dermatitis, and delayed- type hypersensitivity, in arthritic joints, and in heart and renal allografts undergoing rejection.3 E-selectin-targeting antibody drug conjugate has potential as a prostate cancer therapy.4

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Milstone, D. et al. (2004) PNAS 101: 8005
2. Dimitroff, CJ. et al. (2009) J Visualized Experiments
3. Hirata, T. et al. (2005) J Immunol. 175: 8042
4. Ramakrishnan Vanitha et al. (2003) Cancer Res. 63: 6387
IHC FFPE
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.