Anti-Mouse E-Selectin (Clone 96403)
Anti-Mouse E-Selectin (Clone 96403)
Product No.: E255
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Clone 96403 Target E-Selectin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SELE, CD62E, Endothelial Leukocyte Adhesion Molecule (ELAM), ELAM1, ESEL, LECAM2, CD62E Isotype IgG2a Applications WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse E-Selectin Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830027 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse E-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse E-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse E-Selectin (Clone 96403) recognizes an epitope on Mouse E-Selectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background E-selectin, also known as CD62E is an inducible leukocyte adhesion glycoprotein specifically expressed by endothelial cells.1 E-selectin helps initiate recruitment of circulating leukocytes to cutaneous, bone and inflamed tissues.2 E-selectin is found in inflammatory skin lesions in psoriasis, contact dermatitis, and delayed- type hypersensitivity, in arthritic joints, and in heart and renal allografts undergoing rejection.3 E-selectin-targeting antibody drug conjugate has potential as a prostate cancer therapy.4 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Milstone, D. et al. (2004) PNAS 101: 8005 2. Dimitroff, CJ. et al. (2007) Expert Opin Ther Targets. 11(11):1473-91. 3. Hirata, T. et al. (2005) J Immunol. 175: 8042 4. Vanitha, R. et al. (2003) Cancer Res. 63: 6387 Technical Protocols |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
