Anti-Mouse E-Selectin (Clone 96403)

Anti-Mouse E-Selectin (Clone 96403)

Product No.: E255

[product_table name="All Top" skus="E255"]

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SELE, CD62E, Endothelial Leukocyte Adhesion Molecule (ELAM), ELAM1, ESEL, LECAM2, CD62E

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Antibody Details

Product Details

Reactive Species
Host Species
NS0-Derived Recombinant Mouse E-Selectin Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse E-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse E-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Rat Anti-Mouse E-Selectin (Clone 96403) recognizes an epitope on Mouse E-Selectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
E-selectin, also known as CD62E is an inducible leukocyte adhesion glycoprotein specifically expressed by endothelial cells.1 E-selectin helps initiate recruitment of circulating leukocytes to cutaneous, bone and inflamed tissues.2 E-selectin is found in inflammatory skin lesions in psoriasis, contact dermatitis, and delayed- type hypersensitivity, in arthritic joints, and in heart and renal allografts undergoing rejection.3 E-selectin-targeting antibody drug conjugate has potential as a prostate cancer therapy.4

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Milstone, D. et al. (2004) PNAS 101: 8005
2. Dimitroff, CJ. et al. (2007) Expert Opin Ther Targets. 11(11):1473-91.
3. Hirata, T. et al. (2005) J Immunol. 175: 8042
4. Vanitha, R. et al. (2003) Cancer Res. 63: 6387
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.