Anti-Mouse Fractalkine (Clone 126315)

Product No.: C1442


Clone 126315 Target Fractalkine Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CX3CL1, NTN, ABCD-3, C3Xkine, CXC3, CXC3C, NTT, SCYD1, Neurotactin, FKN Isotype IgG2a Applications ELISA Cap , N , WB


Select Product Size 100 µg — $500.00 25 µg — $250.00 500 µg — $0	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse FKN (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828504 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:F1098 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:F1035. Western Blotting: To detect Mouse FKN this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FKN is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1035. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse CX3CL1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CX3CL1 (at 30 ng/ml) is 0.75 - 3.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse CX3CL1 (Clone 126315) recognizes an epitope on Mouse CX3CL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody displayed 100% cross-reactivity with human CX3CL1 chemokine domain. Other chemokines tested 1 displayed negligible cross-reactivity. Background Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium¹ that mediates attachment and firm adhesion of T cells, monocytes and NK cells.² FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.³ FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).⁴ PubMed Fractalkine NCBI Gene Bank ID 6376 UniProt.org Information on Uniprot.org References & Citations 1. Patel, DD. et al. (1998) J Exp Med. 188: 1413 2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551 3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424 4. Mori, M. et al. (2005) Int J Oncol. 26: 41 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

Purified Recombinant Mouse FKN (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828504

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:F1098 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:F1035.
Western Blotting: To detect Mouse FKN this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FKN is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1035.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Mouse CX3CL1 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Mouse CX3CL1 (at 30 ng/ml) is 0.75 - 3.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CX3CL1 (Clone 126315) recognizes an epitope on Mouse CX3CL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody displayed 100% cross-reactivity with human CX3CL1 chemokine domain. Other chemokines tested 1 displayed negligible cross-reactivity.

Background

Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium¹ that mediates attachment and firm adhesion of T cells, monocytes and NK cells.² FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.³ FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).⁴

PubMed

Fractalkine

NCBI Gene Bank ID

6376

UniProt.org

Information on Uniprot.org

References & Citations

1. Patel, DD. et al. (1998) J Exp Med. 188: 1413
2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551
3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424
4. Mori, M. et al. (2005) Int J Oncol. 26: 41

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
C1406	Anti-Human Fractalkine (Clone 81513)
C1405	Anti-Human Fractalkine (Clone 51637)
C1442	Anti-Mouse Fractalkine (Clone 126315)
C1306	Anti-Human Fractalkine
C1438	Anti-Rat Fractalkine (Clone 96834)
F1069	Anti-Mouse Fractalkine
C1467	Anti-Rat Fractalkine
F1038	Recombinant Rat Fractalkine (Chemokine Domain, aa 25-100)
F1023	Recombinant Rat Fractalkine (FKN)
F1115	Recombinant Rat Fractalkine
F1035	Recombinant Mouse Fractalkine
F1036	Recombinant Mouse Fractalkine (FKN)
F136	Anti-Human Fractalkine – Biotin
C1512	Anti-Rat Fractalkine – Biotin
F1098	Anti-Mouse Fractalkine – Biotin

Formats Available

Prod No.	Description
C1442	Anti-Mouse Fractalkine (Clone 126315)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

NEW Products!

Anti-Mouse Fractalkine (Clone 126315)