Anti-Mouse Fractalkine – Biotin

Pricing & Details

Product No.F1098
Protein
Fractalkine
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
CX3CL1, NTN, ABCD-3, C3Xkine, CXC3, CXC3C, NTT, SCYD1, Neurotactin, FKN
Applications
ELISA Det
,
WB
Prod No.
Size
Price
Avail.
Qty
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F1098-50 µg
50 µg
$510.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse FKN (O35188) (Accession # O35188)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse FKN this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FKN is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:F1035.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:C1442 as the capture antibody at 4 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse FKN.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Fractalkine recognizes Mouse Fractalkine. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium1 that mediates attachment and firm adhesion of T cells, monocytes and NK cells.2 FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.3 FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).4

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Patel, DD. et al. (1998)J Exp Med. 188: 1413
2. Hwang, ST. et al. (1999)Eur J Immunol. 29: 2551
3. Muller, GA. et al. (2008)Z Rheumatol. 67: 424
4. Mori, M. et al. (2005)Int J Oncol. 26: 41
Indirect Elisa Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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