Anti-Mouse H-2K^d (MHC Class I) – Purified in vivo GOLD™ Functional Grade

Clone A4C8.1-Do9 is used in in vivo mouse studies as a monoclonal antibody that specifically targets MHC Class I H-2Kd molecules on mouse cells.

This antibody is utilized primarily to:

• Bind to and block MHC Class I H-2Kd molecules: This can be used for functional studies of immune modulation, such as depleting CD8+ T cell responses, interfering with antigen presentation, or investigating the role of MHC Class I in disease models.
• Facilitate transplantation, autoimmune, or immunology experiments: By blocking or neutralizing MHC Class I, researchers can analyze how disruption of these molecules influences immune rejection, tolerance, or infection.

Key features:

• Applications: While often used in western blotting (WB), the “In Vivo” label and low endotoxin preparation make it suitable for injection into live mice, minimizing inflammatory side effects.
• Specificity: Recognizes H-2Kd, which is expressed on cells from mice with the appropriate haplotype (excluding red blood cells); relevant for strains such as BALB/c.
• Usage notes: Each investigator must optimize the dosing and schedule for their experimental needs.

Summary table:

These antibodies are strictly for research use only and not for diagnostic or therapeutic applications. The exact protocol (e.g., dose, route, timing) varies according to experimental design, so investigators need to calibrate usage according to their model requirements.

The correct storage temperature for sterile packaged monoclonal antibodies like clone A4C8.1-Do9 depends on whether you are prioritizing sterility (general handling of sterile packaged items) or antibody stability and activity (specific to the monoclonal antibody content).

• For monoclonal antibodies’ stability:
  Most sterile monoclonal antibodies should be stored at 2–8°C (refrigerator temperature) for short-term use, and for long-term storage, at -20°C to -80°C is recommended to maintain efficacy and prevent degradation. Do not subject them to repeated freeze-thaw cycles, and aliquot if necessary to avoid this.

• For maintaining sterility of the packaging:
  If you are referring to the storage environment for sterilized packaged products (not specific to antibodies), best practices call for temperature between 18°C and 23°C with relative humidity of 30–60% to maintain package integrity and sterility. However, this does not reflect requirements for biological activity, only for sterility and packaging shelf life.

Summary Table:

For clone A4C8.1-Do9, unless manufacturer documentation specifies a unique requirement, follow standard monoclonal antibody guidance: store at 2–8°C for short term, -20°C or lower for long term. If only sterility is the concern (such as unopened, non-biological implantables), use the 18–23°C range. Always check the datasheet or label for clone-specific instructions.

A4C8.1-Do9 is a monoclonal antibody, though the search results do not specify its target or application, so this answer is based on standard antibody co-utilization practices in immunology, especially as suggested by the provided literature.

In studies utilizing antibodies like A4C8.1-Do9, researchers commonly use the following antibodies and proteins alongside it to investigate immune responses, tissue environments, or co-localized antigens:

• F4/80: A marker for macrophages, often co-stained to assess immune cell infiltrates in tissues.
• Ly6C: Used to distinguish monocyte subpopulations in tissue or circulating blood.
• IL-1?: Important cytokine, often stained to assess inflammatory responses and cellular activation.
• XCR1: A marker for a subset of dendritic cells involved in antigen presentation, often visualized to study immune cell clustering.
• CD8 and CD4: For identifying cytotoxic or helper T cell subsets within immune responses.
• Antibodies against AAV capsid or transgene product: Often used in gene therapy or immunology research to monitor antigen-specific T cell responses.
• Secondary antibodies: Species-specific, conjugated with fluorescent dyes or enzymes for signal detection in immunofluorescence (IF) or immunohistochemistry (IHC).
• Other cell markers: Such as CD40/CD40L (co-stimulatory interactions), MyD88 (innate immune signaling), and various cytokines for the functional study of cell pathways.

These antibodies are typically chosen to allow multiplex detection of different cell types or proteins:

• By selecting antibodies from different host species (e.g., mouse, rabbit), researchers avoid cross-reactivity when using multiple monoclonals in the same experiment.
• Using distinct fluorochromes or chromogenic substrates ensures clear, simultaneous visualization of target antigens.
• Controls such as isotype-matched antibodies also play an essential role in validating specificity.

The co-use of F4/80, Ly6C, IL-1?, XCR1, and CD8/CD4 antibodies is particularly common in studies analyzing immune cell dynamics in tissues—such as liver following gene therapy protocols—where cellular infiltration, activation, and co-localization are critical outcomes.

If a specific protein or antigen context for A4C8.1-Do9 is needed, providing its target or application would allow for a more precise answer. Otherwise, these listed antibodies represent the standard panel of immune markers often used in tandem with application-specific monoclonals in immunological and tissue-based research.

Clone A4C8.1-Do9 is a monoclonal antibody that specifically recognizes the major histocompatibility complex (MHC) class I H-2K^d haplotype in mice, which is ubiquitously expressed on nucleated cells of this genotype.

Key findings and characteristics from scientific citations include:

• Specificity: Clone A4C8.1-Do9 is specific for an epitope on mouse MHC class I H-2K^d, distinguishing cells from strains such as BALB/c (H-2K^d haplotype), and is used to identify and characterize these cells in immunological studies.
• Applications: It is widely used in immunological assays including flow cytometry, ELISA, and Western blotting to label and quantify cells expressing H-2K^d.
• Antibody Details:
  • Monoclonal, mouse origin, isotype IgG1 ?.
  • Available conjugated to allophycocyanin (APC) or as a low-endotoxin, functional-grade antibody for in vivo and in vitro applications.
  • Typical concentration for staining cells in flow cytometry is ?0.25 ?g per (10^6) cells in 100 ?l.
• Handling: Stable for short-term storage at 2–8°C; for long-term, aliquot and store at -80°C to avoid freeze-thaw cycles.
• Biological Context: MHC class I molecules such as H-2K^d mediate antigen presentation to cytotoxic T cells and are a critical target for immunological research involving transplant models, immunophenotyping, and studies of immune cell activation.
• Research Utility: Clone A4C8.1-Do9 provides a reliable tool for distinguishing between mouse strains based on MHC haplotypes, tracking donor cell populations, and studying immune responses in experimental models.

No significant controversies or conflicting results are reported in the cited literature. Clone A4C8.1-Do9 is recognized as a standard reagent in murine immunology research for analysis of H-2K^d expression.