Anti-Mouse H-2Kd (MHC Class I) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse H-2Kd (MHC Class I) – Purified in vivo GOLD™ Functional Grade
Product No.: H146
Clone A4C8.1-Do9 Target MHC Class I Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Class I major histocompatibility antigen H-2Kd Isotype Mouse IgG1 κ Applications FC , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737514 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone A4C8.1-Do9 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone A4C8.1-Do9 recognizes an epitope on mouse MHC class I H-2K haplotype d. Background H-2Kd antibody, clone A4C8.1-Do9, recognizes the major histocompatibility complex (MHC) class I H-2K haplotype d (H-2Kd). MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2Kd plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11. Antigen Distribution H-2Kd is ubiquitously expressed on nucleated cells from mice of the H-2Kd haplotype, including BALB/c. Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone A4C8.1-Do9 is used in in vivo mouse studies as a monoclonal antibody that specifically targets MHC Class I H-2Kd molecules on mouse cells. This antibody is utilized primarily to:
Key features:
Summary table:
These antibodies are strictly for research use only and not for diagnostic or therapeutic applications. The exact protocol (e.g., dose, route, timing) varies according to experimental design, so investigators need to calibrate usage according to their model requirements. A4C8.1-Do9 is a monoclonal antibody, though the search results do not specify its target or application, so this answer is based on standard antibody co-utilization practices in immunology, especially as suggested by the provided literature. In studies utilizing antibodies like A4C8.1-Do9, researchers commonly use the following antibodies and proteins alongside it to investigate immune responses, tissue environments, or co-localized antigens:
These antibodies are typically chosen to allow multiplex detection of different cell types or proteins:
The co-use of F4/80, Ly6C, IL-1?, XCR1, and CD8/CD4 antibodies is particularly common in studies analyzing immune cell dynamics in tissuessuch as liver following gene therapy protocolswhere cellular infiltration, activation, and co-localization are critical outcomes. If a specific protein or antigen context for A4C8.1-Do9 is needed, providing its target or application would allow for a more precise answer. Otherwise, these listed antibodies represent the standard panel of immune markers often used in tandem with application-specific monoclonals in immunological and tissue-based research. Clone A4C8.1-Do9 is a monoclonal antibody that specifically recognizes the major histocompatibility complex (MHC) class I H-2K^d haplotype in mice, which is ubiquitously expressed on nucleated cells of this genotype. Key findings and characteristics from scientific citations include:
No significant controversies or conflicting results are reported in the cited literature. Clone A4C8.1-Do9 is recognized as a standard reagent in murine immunology research for analysis of H-2K^d expression. Dosing regimens for clone A4C8.1-Do9 primarily depend on the application and mouse model, but available guidance is specific for in vitro use, especially flow cytometry: the recommended concentration is ??0.25??g per 10^6 cells in a 100??L volume. Most published information relates to use for cell staining in flow cytometry rather than in vivo experiments. For flow applications (all mouse models):
For in vivo dosing (e.g., systemic administration or functional studies), no standard regimen for A4C8.1-Do9 is available in the search results as of the latest guidance. For other functional-grade antibodies (used for depletion or functional blockade), doses typically range from 100–300??g per mouse per dose given intraperitoneally, 1–3 times per week, depending on the target and the effect sought. However, this should not be extrapolated to A4C8.1-Do9 without further validation, as the antibody is reported only for in vitro applications. Key considerations for mouse model differences:
If using A4C8.1-Do9 for functional in vivo studies, it is essential to consult lot-specific datasheets and titrate accordingly, as the manufacturer notes that each investigator must determine the optimal working dilution for their specific context. Summary Table: A4C8.1-Do9 Dosing Guidance
No specific regimen differing by mouse model is documented; empirical titration remains the standard approach for optimizing use of this clone in different systems. References & Citations1. Mitaksov V & Fremont DH. S (2006) J Biol Chem. 281(15):10618-25 2. Wieczorek M, et al. (2017) Front Immunol. 8:292. 3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9 4. Matsumura M, et al. (1992) Science. 927–34.10.1126/science.1323878 5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162 6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743 7. Cruz FM, et al. (2017) Annu Rev Immunol. 35:149-176 8. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 07(39):16916-16921 9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856 10. Raulet DH. (1994) Adv Immunol. 55:381-421. 11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 1996;64:44-58 Technical ProtocolsCertificate of Analysis |
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