Anti-Mouse H-2Kk – Biotin

Anti-Mouse H-2Kk – Biotin

Product No.: H151

[product_table name="All Top" skus="H151"]

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Clone
B1B8.C2-Ky99
Target
I H-2Kk
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG3 κ
Applications
FC
,
IP
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone B1B8.C2-Ky99 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IP
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Specificity
Mouse Anti-Class I H-2Kk (Clone B1B8.C2-Ky99) recognizes Class I H-2Kk. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Antigen is present on 100% of T-cells, B-cells, erythrocytes, macrophages, cells of endocrine origin including B-cells of Langerhans, etc., also endothelial and epithelial tissues.

Antigen Details

References & Citations

Hauptfeld, V. (1984) Immunogenetics 19:169
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.