Anti-Mouse H-2Kk – Purified

Anti-Mouse H-2Kk – Purified

Product No.: H150

[product_table name="All Top" skus="H150"]

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Clone
B1B8.C2-Ky99
Target
I H-2Kk
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG3 κ
Applications
FC
,
IHC FF
,
IP
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone B1B8.C2-Ky99 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IP
WB
IHC FF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Class I H-2Kk (Clone B1B8.C2-Ky99) recognizes Class I H-2Kk. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Antigen is present on 100% of T-cells, B-cells, erythrocytes, macrophages, cells of endocrine origin including B-cells of Langerhans, etc., also endothelial and epithelial tissues.

Antigen Details

References & Citations

Hauptfeld, V. (1984) Immunogenetics 19:169
Flow Cytometry
IHC FF
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.