This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone B1B8.C2-Ky99 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Class I H-2Kk (Clone B1B8.C2-Ky99) recognizes Class I H-2Kk. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen is present on 100% of T-cells, B-cells, erythrocytes, macrophages, cells of endocrine origin including B-cells of Langerhans, etc., also endothelial and epithelial tissues.
References & Citations
Hauptfeld, V. (1984) Immunogenetics 19:169
Products are for research use only. Not for use in diagnostic or therapeutic procedures.