Anti-Mouse H-2Kb (MHC Class I, Clone Y-3) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse H-2Kb (MHC Class I, Clone Y-3) – Purified in vivo PLATINUM™ Functional Grade

Product No.: Y200

[product_table name="All Top" skus="Y100"]

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MHC Class I
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
H-2K, H-2 class I histocompatibility antigen, K-B alpha chain, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin
IgG2b κ
in vivo

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Antibody Details

Product Details

Reactive Species
Host Species
Con A stimulated spleen cells from BALB.B mice
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
≥98% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Additional Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Clone Y-3 recognizes an epitope on mouse MHC class I H-2K haplotypes b, k, q, r, s, but not d.
Antigen Distribution
H-2K is ubiquitously expressed on nucleated cells.
H-2K antibody, clone Y-3, recognizes the major histocompatibility complex (MHC) class I H-2K haplotypes b, k, q, r, s, but not d. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2K plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.

Antigen Details

Research Area
Innate Immunity

References & Citations

1. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25.
2. Wieczorek M, et al. (2017) Front Immunol. 8:292.
3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9.
4. Matsumura M, et al. Science (1992) 257:927–34.10.1126/science.1323878
5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162
6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743
7. Cruz FM, et al. (2017) Annu Rev Immunol. 35:149-176.
8. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921.
9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856.
10. Raulet DH. (1994) Adv Immunol. 55:381-421.
11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.