Anti-Mouse/Human CD45R (B220) [Clone RA3-6B2] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Human CD45R (B220) [Clone RA3-6B2] — Purified in vivo GOLD™ Functional Grade
Product No.: C383
Clone RA3-6B2 Target CD45R Formats AvailableView All Product Type Monoclonal Antibody Alternate Names B220 Isotype Rat IgG2a κ Applications Act , CyTOF® , Depletion , FA , FC , IHC FF , in vivo , IP , PhenoCycler® |
Data
Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Abelson murine leukemia virus-induced pre-B tumor cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737486 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this RA3-6B2 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? Act CODEX® CyTOF® Depletion FA IHC (Frozen) IHC (Paraffin) IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone RA3-6B2 recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule. Background CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform. Antigen Distribution The CD45R antigen is present on mouse B-cells, B-cell precursors and lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC restricted CTL). Ligand/Receptor Galectin-1, CD2, CD3, CD4 Function Phosphatase, T and B cell activation PubMed UniProt.org Research Area Cell Biology . Immunology . Inhibitory Molecules . Neuroscience . Neuroscience Cell Markers Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Common In Vivo Applications of Clone RA3-6B2 in MiceClone RA3-6B2 is widely used in immunology research for its ability to target the CD45R/B220 isoform, which is predominantly expressed on mouse B cells. Here are some common in vivo applications of this clone:
Overall, clone RA3-6B2 is a versatile tool in mouse models for studying B cell biology and immune regulation in vivo. To profile immune cell populations in the literature, especially in mouse immunology, the RA3-6B2 antibody (specific for CD45R/B220, a pan–B cell marker) is frequently paired with antibodies targeting other key cell surface proteins to enable comprehensive identification and characterization of immune subsets. Commonly Paired Antibodies and Proteins
Additional Context
Typical Multicolor Flow Cytometry PanelsA typical mouse lymphocyte panel might include:
These combinations allow researchers to accurately identify, subset, and analyze B cell populations and to distinguish them from other immune lineages. SummaryRA3-6B2 is most commonly used in combination with CD19, CD3, CD4, CD8, NK1.1, CD11b, IgM, IgD, CD21, and CD23 in the literature to thoroughly characterize immune cell populations, especially in mouse models. These combinations are standard in flow cytometry and immunohistochemistry panels for comprehensive immune phenotyping. Clone RA3-6B2 is one of the most widely used monoclonal antibodies targeting the CD45R/B220 isoform and is heavily cited in scientific literature for identifying and characterizing mouse B cells, as well as certain T cell and NK cell populations. Key findings and applications from RA3-6B2 citations include:
Overall, the RA3-6B2 clone is heavily cited for its critical role as a pan-B-cell marker in murine models, its utility in immune modulation, and its centrality in studies of hematopoietic and immune system biology. Dosing regimens for clone RA3-6B2, a monoclonal antibody targeting mouse CD45R/B220, vary significantly depending on the experimental purpose, mouse strain, and application, but for flow cytometry, the recommended dose is typically ≤0.5 µg per test, while for in vivo modulation, the dose and frequency must be empirically determined for each model.
In summary, clone RA3-6B2 is most commonly used at ≤0.5 µg/test for flow cytometry, but in vivo regimens require empirical optimization depending on mouse strain and experimental goals. No standardized dosing exists for in vivo depletion or modulation: researchers must titrate for their specific mouse model and desired outcome. References & Citations1.) Coffman, B. et al. (1982) Immunological Rev. 69:5 2.) Zuhair, K. et al. (1993) J. Immunol. 150:17 3.) Asensi, V. et al. (1989) Immunology 68:204 4.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.


