Anti-Mouse/Human CD45R (B220) [Clone RA3-6B2] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Human CD45R (B220) [Clone RA3-6B2] — Purified in vivo GOLD™ Functional Grade
Product No.: C383
Clone RA3-6B2 Target CD45R Formats AvailableView All Product Type Monoclonal Antibody Alternate Names B220 Isotype Rat IgG2a κ Applications Act , CyTOF® , Depletion , FA , FC , IHC FF , in vivo , IP , PhenoCycler® |
Data
Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Abelson murine leukemia virus-induced pre-B tumor cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737486 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this RA3-6B2 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? Act CODEX® CyTOF® Depletion FA IHC (Frozen) IHC (Paraffin) IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone RA3-6B2 recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule. Background CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform. Antigen Distribution The CD45R antigen is present on mouse B-cells, B-cell precursors and lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC restricted CTL). Ligand/Receptor Galectin-1, CD2, CD3, CD4 Function Phosphatase, T and B cell activation PubMed UniProt.org Research Area Cell Biology . Immunology . Inhibitory Molecules . Neuroscience . Neuroscience Cell Markers Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone RA3-6B2 is a monoclonal antibody targeting mouse CD45R (also known as B220), and in in vivo mouse studies, it is used primarily to deplete or modulate B cells and influence immune cell differentiation and function. Key uses and details in in vivo studies:
Mechanism: The antibody binds to an extracellular epitope of the CD45R molecule (predominantly B cells, but also certain T and NK cell subsets), leading to cell depletion via Fc-dependent mechanisms or modulating their response and function in vivo. Experimental applications:
References and usage precautions:
In summary, clone RA3-6B2 is widely used for in vivo B cell depletion and immunomodulation in mouse models, and plays a critical role in elucidating B cell functions and immune regulation. The correct storage temperature for sterile packaged clone RA3-6B2 (anti-mouse CD45R/B220 monoclonal antibody) is 2–8°C, protected from light and do not freeze. This is the standard condition for the majority of sterile, fluorochrome-conjugated or unconjugated RA3-6B2 antibody preparations.
RA3-6B2 is a monoclonal antibody widely used for detecting the CD45R/B220 isoform, primarily as a murine B cell marker. In the literature, RA3-6B2 is frequently used in combination with the following antibodies or proteins, depending on the experimental context:
These combinations are common in multicolor flow cytometry panels, immunohistochemistry, and immunofluorescence assays to characterize lymphocyte subsets in mouse models. Specific references and applications, such as spatial biology and tissue staining, are also documented. In summary, antibodies against CD19, CD3, CD4, CD8, NK1.1, CD11b, IgM, IgD, CD21, and CD23 are among the most frequently paired with RA3-6B2 in the literature to thoroughly profile immune cell populations. Clone RA3-6B2 is principally recognized in scientific literature as a monoclonal antibody specific for the CD45R/B220 isoform of CD45, and it is extensively used as a marker for B cells, certain T cell subsets, and NK cell subsets in both mouse and, less commonly, human research. Key findings from its citations highlight the following:
In summary, RA3-6B2s citations support its central role as a B cell marker and tool in immunological research, contributing to the understanding of immune cell identity, differentiation, and signaling pathways critical for adaptive immunity. References & Citations1.) Coffman, B. et al. (1982) Immunological Rev. 69:5 2.) Zuhair, K. et al. (1993) J. Immunol. 150:17 3.) Asensi, V. et al. (1989) Immunology 68:204 4.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link Technical ProtocolsCertificate of Analysis |
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