Anti-Mouse Lymphotactin (Clone 80222)
Anti-Mouse Lymphotactin (Clone 80222)
Product No.: X114
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Clone 80222 Target Lymphotactin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYC1, LTN, LPTN, ATAC, SCM-1a, SCM-1, XCL1, Lptn Isotype IgG2a Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse Lymphotactin (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832191 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 4 µg/ml. A suitable detection antibody is PN:L229 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:L196. Western Blotting: To detect Mouse Lymphotactin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Lymphotactin is 50 and 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L196. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Lymphotactin (Clone 80222) recognizes an epitope on Mouse Lymphotactin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Lymphotactin (LTN) is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells.1 LTN plays an important role in maintaining the integrity of the epithelium and in mucosal immune responses.2 Lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection. Antigen DetailsPubMed References & Citations1. Saucedo-Cardenas, O. et al. (2009) Biotechnol. Lett. 31: 215 2. McGhee, JR. et al. (1999) J. Immunol. 162: 1959 Technical Protocols  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
