Purified Recombinant Mouse Lymphotactin (>98%)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse Lymphotactin this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Lymphotactin is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L196.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL. in combination with: Capture Reagent: Mouse XCL1/Lymphotactin Antibody. Standard: Recombinant Mouse XCL1/Lymphotactin Protein.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2 - 15 µg/ml. The recommended secondary antibody for IHC is PN:A106. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Mouse Lymphotactin (Lptn) recognizes Mouse Lptn. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Lymphotactin (LTN) is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells.1 LTN plays an important role in maintaining the integrity of the epithelium and in mucosal immune responses.2 Lymphotactin is a key regulator of lymphocyte motility and adhesion during acute allograft rejection.
NCBI Gene Bank ID
References & Citations
1. Saucedo-Cardenas, O. et al. (2009) Biotechnol. Lett. 31: 215
2. McGhee, JR. et al. (1999) J. Immunol. 162: 1959
Products are for research use only. Not for use in diagnostic or therapeutic procedures.