Anti-Mouse PD-L1- Purified In vivo PLATINUM™ Functional Grade

Pricing & Details

Clone
10F.9G2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Programmed Death Ligand 1, B7-H1, PD-L1, CD274
Isotype
Rat
IgG2b κ
Prod No.
Size
Price
Avail.
Qty
Add to cart
P371-1.0 mg
1.0 mg
$198.00
In stock
P371-5.0 mg
5.0 mg
$355.00
In stock
P371-25 mg
25 mg
$995.00
In stock
P371-50 mg
50 mg
$1,500.00
In stock
P371-100 mg
100 mg
$2,220.00
In stock

Antibody Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
UnKown
Product Concentration
≥ 2.0 mg/ml
Formulation
This Purified Functional Grade, PLATINUM antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl and does not contain BSA or sodium azide as a preservative.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Description

Specificity
Clone 10F.9G2 recognizes an epitope on mouse PD-L1.
Antigen Distribution
PD-L1 is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.
Background
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.

Antigen Details

Protein Family
PD-L1
Ligand/Receptor
PD-1 (PDCD1)
NCBI Gene Bank ID

References & Citations

1. Freeman G, et al. 2000. J. Exp. Med. 192:1027.
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.