Anti-Mouse PD-L1 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse PD-L1 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: P502

[product_table name="All Top" skus="P363"]

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Clone
10F.9G2
Target
PD-L1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B7-H1, CD274
Isotype
IgG2b κ
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone 10F.9G2 has been validated for use on CODEX® using mouse T-cell Sarcoma fresh frozen (FF) tissue.
Tissue Screened
T-cell Sarcoma
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
Unknown
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This PDL-1 (Clone 10F.9G2) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892954
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 10F.9G2 recognizes an epitope on mouse PD-L1.
Antigen Distribution
PD-L1 is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.
Background
PD-1 is a 50-55 kD member of the B7 Ig superfamily. PD-1 is also a member of the extended CD28/CTLA-4 family of T cell regulators and is suspected to play a role in lymphocyte clonal selection and peripheral tolerance. The ligands of PD-1 are PD-L1 and PD-L2, and are also members of the B7 Ig superfamily. PD-1 and its ligands negatively regulate immune responses. PD-L1, or B7-Homolog 1, is a 40 kD type I transmembrane protein that has been reported to costimulate T cell growth and cytokine production. The interaction of PD-1 with its ligand PD-L1 is critical in the inhibition of T cell responses that include T cell proliferation and cytokine production. PD-L1 has increased expression in several cancers. Inhibition of the interaction between PD-1 and PD-L1 can serve as an immune checkpoint blockade by improving T-cell responses In vitro and mediating preclinical antitumor activity. Within the field of checkpoint inhibition, combination therapy using anti-PD1 in conjunction with anti-CTLA4 has significant therapeutic potential for tumor treatments. PD-L2 is a 25 kD type I transmembrane ligand of PD-1. Via PD-1, PD-L2 can serve as a coinhibitor of T cell functions. Regulation of T cell responses, including enhanced T cell proliferation and cytokine production, can result from mAbs that block the PD-L2 and PD-1 interaction.

Antigen Details

Protein
Ligand/Receptor
PD-1 (PDCD1)
PubMed
NCBI Gene Bank ID

References & Citations

1. Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117.
IHC
PhenoCycler®
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.