Anti-Mouse TIGIT [Clone 1B4] — CF®647

Recombinant murine TIGIT tetramers.

This CF647 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, containing 0.09% sodium azide as a preservative.

Liquid

This antibody conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

CF647 is designed for use with 633 or 635 nm laser lines, this dye serves as a highly effective alternative to Cy®5, Alexa Fluor® 647, and DyLight® 650.

FA,
FC,
ELISA

1B4 activity is directed against mouse TIGIT.

TIGIT is an immune checkpoint receptor that inhibits critical immune responses, including T cell priming by dendritic cells and the tumor-killing activity of NK and cytotoxic T cells. Because it also enhances the suppressive function of regulatory T cells, TIGIT has emerged as a significant target for cancer immunotherapy, often in combination with the PD-1/PD-L1 pathway. By conjugating the 1B4 monoclonal antibody to the CF®647 fluorophore, researchers can effectively label TIGIT-expressing cells for ex vivo analysis. This combination is particularly beneficial for high-sensitivity flow cytometry, as the bright, photostable signal of CF®647 allows for the precise identification and functional assessment of TIGIT-positive populations—such as activated and memory T cells—within complex biological samples.

TIGIT is expressed on NK cells, activated T cells, memory T cells, and a subset of regulatory T cells.

CD155 (PVR) and CD112 (PVRL2)

Q495A1

1 Harjunpää H, Guillerey C. Clin Exp Immunol. 200(2):108-119. 2020.
2 Boles KS, Vermi W, Facchetti F, et al. Eur J Immunol. 39(3):695-703. 2009.
3 Dixon KO, Schorer M, Nevin J, et al. J Immunol. 200(8):3000-3007. 2018.
4 Ozmadenci D, Shankara Narayanan JS, Andrew J, et al. Proc Natl Acad Sci U S A. 119(17):e2117065119. 2022.