Anti-Mouse TIGIT [Clone 1B4] — CF®700

Recombinant murine TIGIT tetramers.

This CF®700 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 0.09% sodium azide as a preservative.

Liquid

This antibody conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

Optimized for 633 nm, 635 nm 680 nm or 685 nm red/near-infrared (NIR) laser Lines.

FA,
FC,
ELISA

1B4 activity is directed against mouse TIGIT.

TIGIT is an immunoreceptor that functions as an immune checkpoint, inhibiting essential responses such as T cell priming by dendritic cells and the tumor-killing capacity of NK and cytotoxic T cells, while simultaneously enhancing the suppressive activity of regulatory T cells. Given its role in suppressing anti-tumor immunity, TIGIT is a prominent target for cancer therapy, particularly when modulated alongside the PD-1/PD-L1 pathway to promote tumor suppression.

NOTE: To facilitate the study of this target in in vivo and live animal models, the 1B4 antibody can be conjugated to CF®700, CF®750, or Leinco NIR-SHEILD aa high-performance near-infrared (NIR) dye. Utilizing a NIR probe's is highly beneficial for tumor imaging because the longer emission wavelengths minimize tissue autofluorescence and maximize light penetration depth in live specimens, allowing for more accurate, non-invasive tracking of TIGIT-expressing cell populations within the tumor microenvironment.

TIGIT is expressed on NK cells, activated T cells, memory T cells, and a subset of regulatory T cells.

CD155 (PVR) and CD112 (PVRL2)

Q495A1

1 Harjunpää H, Guillerey C. Clin Exp Immunol. 200(2):108-119. 2020.
2 Boles KS, Vermi W, Facchetti F, et al. Eur J Immunol. 39(3):695-703. 2009.
3 Dixon KO, Schorer M, Nevin J, et al. J Immunol. 200(8):3000-3007. 2018.
4 Ozmadenci D, Shankara Narayanan JS, Andrew J, et al. Proc Natl Acad Sci U S A. 119(17):e2117065119. 2022.