Anti-Mouse TIGIT [Clone 1B4] — HRP

Recombinant murine TIGIT tetramers.

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Liquid

This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2 – 8° C Wet Ice

FA,
FC,
ELISA

1B4 activity is directed against mouse TIGIT.

TIGIT is an immunoreceptor that inhibits multiple immune cell responses, including T cell priming by dendritic cells, tumor cell killing by NK cells and cytotoxic T cells, and also enhances the immune suppressive activity of regulatory T cells¹. TIGIT is a novel member of the Ig-superfamily distantly related to Nectins and Necls that aligns with the distal Ig-V-type domains of Nectin1-4, poliovirus receptor (PVR; CD155), DNAM-1 (CD226), and TACTILE (CD96)². TIGIT is an attractive target for cancer therapy due to its role as an immune checkpoint^1,3. Immunotherapy targeting TIGIT and the PD-1/PD-L1 pathway is capable of tumor suppression.

1B4 was generated by immunizing TIGIT - / - mice with recombinant mouse TIGIT tetramers³. Draining lymph nodes were collected, cells fused with Sp2/0-Ag14, supernatants screened for specific binding by anti-TIGIT ELISA and flow cytometry, and ultimately binding specificity was confirmed by staining wild-type, activated, primary TIGIT-expressing T cells.

TIGIT is expressed on NK cells, activated T cells, memory T cells, and a subset of regulatory T cells.

CD155 (PVR) and CD112 (PVRL2)

Q495A1

1 Harjunpää H, Guillerey C. Clin Exp Immunol. 200(2):108-119. 2020.
2 Boles KS, Vermi W, Facchetti F, et al. Eur J Immunol. 39(3):695-703. 2009.
3 Dixon KO, Schorer M, Nevin J, et al. J Immunol. 200(8):3000-3007. 2018.
4 Ozmadenci D, Shankara Narayanan JS, Andrew J, et al. Proc Natl Acad Sci U S A. 119(17):e2117065119. 2022.