Anti-Mouse CD120b (TNFR2) [Clone TR75-54.7] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD120b (TNFR2) [Clone TR75-54.7] — Purified in vivo PLATINUM™ Functional Grade
Product No.: T954
Clone TR75-54.7 Target TNFR2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Tumor Necrosis Factor Receptor II, p75, CD120b, TBPII, TNF-R75, TNFBR, TNFR2, TNFR80, p75TNFR Isotype IgG Applications B , ELISA Cap , FC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Recommended Isotype Controls Recommended Dilution Buffer Immunogen Purified Recombinant Mouse TNF R2 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2832125 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone TR75-54.7 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IP B WB ELISA Cap Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Anti-Mouse CD120 recognizes Mouse CD120. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Tumor necrosis factor receptor II (TNF-RII) is present on most cell types1 and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.2 Antigen Distribution Variety of cell types at low levels Ligand/Receptor TNF-α, LT-α (TNF-β) Function Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone TR75-54.7 is most commonly used in vivo in mice to block or study signaling through TNFR2 (CD120b), facilitating research in immunoregulation, tumor immunology, and therapeutic interventions targeting TNF receptor pathways. Key in vivo applications include:
TR75-54.7 is used in other technical applications (like flow cytometry, immunoprecipitation, and ELISA), but in vivo, its roles are predominantly focused on anti-tumor efficacy, immune modulation, and pathway blockade in mouse models. TR75-54.7, an anti-mouse TNFR2 antibody, is commonly paired with several other antibodies and proteins in research applications. The most frequently used companion reagents include: TR75-32.4 is the primary antibody used alongside TR75-54.7, particularly for ELISA applications. TR75-54.7 serves as the capture antibody while biotinylated TR75-32.4 functions as the detecting antibody. This pairing creates an effective sandwich ELISA system for detecting mouse TNFR2. PC61, an anti-CD25 antibody, is frequently combined with TR75-54.7 for in vivo functional studies. This combination has proven particularly effective in cancer immunotherapy research. In mouse 4T1 breast cancer models, the combination of TR75-54.7 and PC61 demonstrated superior antitumor effects compared to either antibody alone, with the combination therapy extending median survival to 38 days compared to 34 days for TR75-54.7 alone or 27 days for PC61 alone. TR75-89 represents another anti-TNFR2 monoclonal antibody that has been investigated alongside TR75-54.7. While both antibodies act as TNFR2 agonists, they differ in their mechanism—TR75-54.7 competes with TNF-α for binding, whereas TR75-89 does not compete with TNF-α. For secondary detection in flow cytometry applications, biotinylated anti-Armenian hamster IgG antibodies are recommended, often followed by streptavidin-conjugated fluorochromes to amplify signal detection. This three-layer staining technique helps maximize signal over background in immunofluorescent staining applications. Clone TR75-54.7 is a monoclonal antibody that targets mouse Tumor Necrosis Factor Receptor Type II (TNFR2), also known as CD120b or p75. Here are the key findings from its citations in scientific literature:
Overall, clone TR75-54.7 is a valuable tool for studying TNFR2's role in immune regulation and cancer therapy, offering both agonist and antagonist activities depending on the context. Dosing regimens for clone TR75-54.7, an anti-mouse TNFR2 antibody, vary across different mouse models primarily based on three key factors: mouse strain, tumor type, and experimental design. Here are some specific considerations:
These variations allow researchers to optimize treatment strategies for different research models, ensuring the most effective outcomes in preclinical studies. Specific dosages and administration schedules can be found in the detailed protocols for each model. For example, in some studies, mice are treated with 100 μg of anti-TNFR2 mAb intraperitoneally at specific intervals after tumor implantation. However, the exact dosing regimen should be determined based on the specific requirements of the model being used. References & Citations1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217. 2. Sheehan, KC. et al. (1995) J. Exp. Med. 181:607. Technical ProtocolsCertificate of Analysis |
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