Anti-PolyHistidine

Pricing & Details

Product No.P246
Clone
AD1.1.10
Protein
PolyHistidine
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG1
Applications
CyTOF®
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
P246-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
P246-100 µg
100 µg
$399.00
In stock
Max:
Min: 1
Step: 1
P246-500 µg
500 µg
$849.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Host Species
Mouse
Immunogen
A polypeptide containing a polyhistidine tag
Endotoxin Level
<0.2 EU/µg as determined by LAL
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect polyhistidine this antibody can be used at a concentration of 0.2 µg/ml. This antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for polyhistidine is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/106 cells
Other Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-PolyHistidine (Clone AD1.1.10) recognizes polyhistidine-tagged polypeptides. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

Flow Cytometry
General Western Blot Protocol

Formats Available

Prod No.
Description
P246
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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