Anti-PolyHistidine (AD1.1.10) – Purified No Carrier Protein

Anti-PolyHistidine (AD1.1.10) – Purified No Carrier Protein

Product No.: P246

[product_table name="All Top" skus="P246"]

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names

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Antibody Details

Product Details

Host Species
A polypeptide containing a polyhistidine tag
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ice Packs
Applications and Recommended Usage?
Quality Tested by Leinco
WB2, 4, 5, 6,
Additional Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


AD1.1.10 activity is directed against proteins and peptides containing the H-H-H-H-H-H (6His) motif.
Antigen Distribution
The 6His motif is present in histidine tags.
AD1.1.10 recognizes proteins and peptides containing the polyhistidine tag (His-tag). The most common His-tag is the H-H-H-H-H-H (6x-His) motif, but His-tags can comprise up to 10 His residues. His-tags can be inserted into a target gene either by cloning the encoding DNA in frame into a His-tag containing vector or by using extended polymerase chain reaction primers that incorporate the motif. His-tags can be inserted at either the N or C terminal of target proteins. The most suitable location depends on the target protein’s characteristics as well as the downstream experimental methods. A variety of purification and detection methods make use of His-tags. Applications include flow cytometry, ELISA, Western blot, dot blot, peptide array, immunohistochemistry, immunofluorescence, immunoprecipitation, immunocytochemistry, histidine tag (His-tag) insertion for epitope mapping (HisMAP), and chromatin immunoprecipitation (ChIP).

His-tags are less likely than other tags to affect functionality of the fusion protein due to their relatively small size. However, His residues exist in mammalian and insect systems, and therefore there may be some background binding, depending on the experimental conditions and/or the system used.

Antigen Details

Research Area
Epitope Tag
Protein Purification

References & Citations

1. Herrera NG, Morano NC, Celikgil A, et al. ACS Omega. 6(1):85-102. 2020.
2. Els Conrath K, Lauwereys M, Wyns L, et al. J Biol Chem. 276(10):7346-7350. 2001.
3. Suen JL, Wu CH, Chen YY, et al. Immunology. 103(3):301-309. 2001.
4. Yi KS, Choi JA, Kim P, et al. PLoS One. 15(7):e0236172. 2020.
5. Lam M, Marsters SA, Ashkenazi A, et al. Elife. 9:e52291. 2020.
6. Moreland KT, Hong M, Lu W, et al. PLoS One. 9(4):e95333. 2014.
7. Smith MH, Rodriguez EH, Weissman JS. J Biol Chem. 289(37):25670-25677. 2014.
8. Sasakura Y, Kanda K, Yoshimura-Suzuki T, et al. Anal Chem. 76(22):6521-6527. 2004.
9. Asano T, Takei J, Furusawa Y, et al. Monoclon Antib Immunodiagn Immunother. 40(6):243-249. 2021.
10. Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, et al. Curr Biol. 31(7):1521-1530.e8. 2021.
Indirect Elisa Protocol
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.