Anti-PolyHistidine (AD1.1.10) – Dylight® 488

Anti-PolyHistidine (AD1.1.10) – Dylight® 488

Product No.: P397

[product_table name="All Top" skus="P246"]

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Clone
AD1.1.10
Target
PolyHistidine
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
His-Tag
Isotype
IgG1
Applications
ELISA
,
FC
,
IF
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Host Species
Mouse
Immunogen
PAX6 transcription factor linked to histidine tag.
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
WB2, 4, 5, 6,
FC1
Additional Applications Reported In Literature ?
ELISA2,
IP3,
IF10
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
AD1.1.10 activity is directed against proteins and peptides containing the H-H-H-H-H-H (6His) motif.
Antigen Distribution
The 6His motif is present in histidine tags.
Background
AD1.1.10 recognizes proteins and peptides containing the polyhistidine tag (His-tag). The most common His-tag is the H-H-H-H-H-H (6x-His) motif, but His-tags can comprise up to 10 His residues. His-tags can be inserted into a target gene either by cloning the encoding DNA in frame into a His-tag containing vector or by using extended polymerase chain reaction primers that incorporate the motif. His-tags can be inserted at either the N or C terminal of target proteins. The most suitable location depends on the target protein’s characteristics as well as the downstream experimental methods. A variety of purification and detection methods make use of His-tags. Applications include flow cytometry, ELISA, Western blot, dot blot, peptide array, immunohistochemistry, immunofluorescence, immunoprecipitation, immunocytochemistry, histidine tag (His-tag) insertion for epitope mapping (HisMAP), and chromatin immunoprecipitation (ChIP).

His-tags are less likely than other tags to affect functionality of the fusion protein due to their relatively small size. However, His residues exist in mammalian and insect systems, and therefore there may be some background binding, depending on the experimental conditions and/or the system used.

Antigen Details

Research Area
Epitope Tag
.
Protein Purification

References & Citations

1. Herrera NG, Morano NC, Celikgil A, et al. ACS Omega. 6(1):85-102. 2020.
2. Els Conrath K, Lauwereys M, Wyns L, et al. J Biol Chem. 276(10):7346-7350. 2001.
3. Suen JL, Wu CH, Chen YY, et al. Immunology. 103(3):301-309. 2001.
4. Yi KS, Choi JA, Kim P, et al. PLoS One. 15(7):e0236172. 2020.
5. Lam M, Marsters SA, Ashkenazi A, et al. Elife. 9:e52291. 2020.
6. Moreland KT, Hong M, Lu W, et al. PLoS One. 9(4):e95333. 2014.
7. Smith MH, Rodriguez EH, Weissman JS. J Biol Chem. 289(37):25670-25677. 2014.
8. Sasakura Y, Kanda K, Yoshimura-Suzuki T, et al. Anal Chem. 76(22):6521-6527. 2004.
9. Asano T, Takei J, Furusawa Y, et al. Monoclon Antib Immunodiagn Immunother. 40(6):243-249. 2021.
10. Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, et al. Curr Biol. 31(7):1521-1530.e8. 2021.
Indirect Elisa Protocol
Flow Cytometry
IF
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.