Anti-RNase L – Purified in vivo GOLD™ Functional Grade
Anti-RNase L – Purified in vivo GOLD™ Functional Grade
Product No.: R6000
Clone 2E9 Target RNaseL Formats AvailableView All Product Type Monoclonal Antibody Alternate Names 2',5'-oligoisoadenylate synthetase-dependent; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; Ribonuclease 4; Ribonuclease L; ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent); ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent); RNase L; PRCA1; RNS4 Isotype Mouse IgG1 κ Applications ELISA , IHC FFPE , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Hamster ⋅ Human ⋅ Primate Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Purified, full-length, recombinant, human RNaseL produced in insect cells. Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831732 Applications and Recommended Usage? Quality Tested by Leinco WB ELISA Additional Applications Reported In Literature ? IHC FFPE Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 2E9 recognizes an epitope on human/hamster/primate RNaseL.
Background RNase L antibody, clone 2E9, recognizes the interferon (IFN)-inducible 2-5A-dependent ribonuclease L (RNase L), an endoribonuclease involved in antiviral signaling. It is widely expressed as a latent monomer in most mammalian tissues1. 2’,5’-oligoadenylate synthetase (OAS) is expressed following viral infection and exposure to IFN2. OAS proteins are activated by dsRNA, resulting in the production of 2’,5’-linked oligoadenylates (2-5A), which bind to latent RNase L, inducing dimerization and activation2. RNase L inhibits viral replication by cleaving viral and ribosomal RNA3,4 and inducing apoptosis in virus-infected cells5,6. RNase L is suggested to have antitumor effects, possibly through its pro-apoptotic activity7, and mutations in the RNASEL gene are associated with prostate cancer risk and progression8,9. Antigen Distribution RNase L is highly expressed in the spleen and thymus, followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes. PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Neuroinflammation . Neuroscience Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Based on the available search results, clone 2E9 refers to multiple different antibodies targeting various proteins, making it challenging to identify specific in vivo applications in mice for a single clone 2E9. However, the search results provide limited direct information about in vivo mouse applications. Identified Clone 2E9 AntibodiesThe search results reveal several distinct antibodies designated as clone 2E9, each targeting different proteins: Anti-Amyloid beta A4 protein (APP) - This rat monoclonal antibody detects multiple isoforms of APP and targets an 11 amino acid sequence in the C-terminal region. The antibody shows reactivity with both mouse and human species, though the search results do not describe specific in vivo mouse applications. Anti-LAT antibody - A mouse monoclonal antibody targeting LAT (Linker for Activation of T cells) validated for immunoprecipitation and Western blot applications. However, no in vivo mouse studies are mentioned in the available information. Anti-pig SLA Class II DR - This mouse monoclonal antibody recognizes swine leukocyte antigen molecules and inhibits mixed lymphocyte reactions. This antibody is specifically designed for porcine applications rather than mouse studies. LimitationsThe search results do not provide detailed information about common in vivo applications of any clone 2E9 antibody specifically in mouse models. While several clone 2E9 antibodies show mouse species reactivity, the available sources primarily describe in vitro applications such as immunoprecipitation, Western blotting, immunofluorescence, and immunohistochemistry rather than in vivo experimental uses in mice. In the literature, several antibodies and proteins are commonly used alongside the 2E9 antibody, depending on the specific application and research area. Here are a few examples:
In addition to these, proteins like actin or tubulin are often used as loading controls in Western blot experiments to ensure that equal amounts of protein are loaded across different samples. Commonly Used Proteins for Controls:
These proteins are typically used in various antibody-based assays to validate the effectiveness and specificity of the primary antibodies like the 2E9 series. Clone 2E9 citations in scientific literature primarily refer to several different monoclonal antibodies, each targeting distinct proteins in diverse research contexts. The main scientific findings relate to antibody specificity, target detection, and their application in disease models and diagnostic assays. Key findings associated with clone 2E9:
Summary table of notable clone 2E9 citations:
When referencing "clone 2E9" in literature, check the specific target and context, as the designation is reused across distinct antibodies for different proteins. Citations consistently report high specificity and reliable detection within their validated applications. The dosing regimens of clone 2E9 across different mouse models are not comprehensively detailed in available literature or dosing guides, and clone 2E9 is associated with several different targets depending on context (e.g., anti-SLA DR in pigs, anti-Tau in neurobiology, anti-beta amyloid). No authoritative dosing recommendations for clone 2E9 in mouse models are explicitly listed in the standard antibody dosing guides for in vivo studies. Available context and related antibodies suggest the following principles:
Summary Table: Reference Dosing for Mouse Monoclonal Antibodies in Vivo
If you have a specific application, target, or disease model for clone 2E9, providing those details may allow for more tailored guidance. In summary, use established dosing regimens for similar monoclonal antibodies as a starting point until model-specific data for clone 2E9 is published. References & Citations1. Zhou A, et al. (2005) J Interferon Cytokine Res. 25(10):595-603 2. Silverman RH. (2007) Cytokine Growth Factor Rev. 18(5-6):381-8 3. Wreschner DH, et al. (1981) Nucleic Acids Res. 9(7):1571-81 4. Cooper DA, et al. (2014) Nucleic Acids Res. 42(8):5202-16 5. Castelli JC, et al. (1997) J Exp Med. 186(6):967-72 6. Zhou A, et al (1997) EMBO J. 16(21):6355-63 7. Xiang Y, et al. (2003) Cancer Res. 63(20):6795-801 8. Meyer MS, et al. (2010) Carcinogenesis. 31(9):1597-603 9. Silverman RH. (2003) Biochemistry. 42(7):1805-12 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
---|---|
R6000 | |
R6010 | |
R6005 |
