Anti-RNase L – Purified

Product No.: R6010

[product_table name="All Top" skus="C163"]

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Clone
2E9
Target
RNaseL
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
2',5'-oligoisoadenylate synthetase-dependent; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; Ribonuclease 4; Ribonuclease L; ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent); ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent); RNase L; PRCA1; RNS4
Isotype
IgG1 κ
Applications
ELISA
,
IHC FFPE
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human/Hamster/Primate
Host Species
Mouse
Immunogen
Purified, full-length, recombinant, human RNaseL produced in insect cells.
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
WB
ELISA
Additional Applications Reported In Literature ?
IHC FFPE
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 2E9 recognizes an epitope on human/hamster/primate RNaseL.
Antigen Distribution
RNase L is highly expressed in the spleen and thymus, followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes.
Background
RNase L antibody, clone 2E9, recognizes the interferon (IFN)-inducible 2-5A-dependent ribonuclease L (RNase L), an endoribonuclease involved in antiviral signaling. It is widely expressed as a latent monomer in most mammalian tissues1. 2’,5’-oligoadenylate synthetase (OAS) is expressed following viral infection and exposure to IFN2. OAS proteins are activated by dsRNA, resulting in the production of 2’,5’-linked oligoadenylates (2-5A), which bind to latent RNase L, inducing dimerization and activation2. RNase L inhibits viral replication by cleaving viral and ribosomal RNA3,4 and inducing apoptosis in virus-infected cells5,6. RNase L is suggested to have antitumor effects, possibly through its pro-apoptotic activity7, and mutations in the RNASEL gene are associated with prostate cancer risk and progression8,9.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Neuroinflammation
.
Neuroscience

References & Citations

1. Zhou A, et al. (2005) J Interferon Cytokine Res. 25(10):595-603
2. Silverman RH. (2007) Cytokine Growth Factor Rev. 18(5-6):381-8
3. Wreschner DH, et al. (1981) Nucleic Acids Res. 9(7):1571-81
4. Cooper DA, et al. (2014) Nucleic Acids Res. 42(8):5202-16
5. Castelli JC, et al. (1997) J Exp Med. 186(6):967-72
6. Zhou A, et al (1997) EMBO J. 16(21):6355-63
7. Xiang Y, et al. (2003) Cancer Res. 63(20):6795-801
8. Meyer MS, et al. (2010) Carcinogenesis. 31(9):1597-603
9. Silverman RH. (2003) Biochemistry. 42(7):1805-12
Indirect Elisa Protocol
IHC FFPE
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.