Anti-RNase L – Purified in vivo GOLD™ Functional Grade
Pricing & Details
Formats AvailableView All
2',5'-oligoisoadenylate synthetase-dependent; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; interferon-induced 2-5A-dependent RNase; Ribonuclease 4; Ribonuclease L; ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent); ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent); RNase L; PRCA1; RNS4
Purified, full-length, recombinant, human RNaseL produced in insect cells.
≥ 5.0 mg/ml
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Other Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone 2E9 recognizes an epitope on human/hamster/primate RNaseL.
RNase L is highly expressed in the spleen and thymus, followed by prostate, testis, uterus, small intestine, colon, and peripheral blood leukocytes.
RNase L antibody, clone 2E9, recognizes the interferon (IFN)-inducible 2-5A-dependent ribonuclease L (RNase L), an endoribonuclease involved in antiviral signaling. It is widely expressed as a latent monomer in most mammalian tissues1. 2’,5’-oligoadenylate synthetase (OAS) is expressed following viral infection and exposure to IFN2. OAS proteins are activated by dsRNA, resulting in the production of 2’,5’-linked oligoadenylates (2-5A), which bind to latent RNase L, inducing dimerization and activation2. RNase L inhibits viral replication by cleaving viral and ribosomal RNA3,4 and inducing apoptosis in virus-infected cells5,6. RNase L is suggested to have antitumor effects, possibly through its pro-apoptotic activity7, and mutations in the RNASEL gene are associated with prostate cancer risk and progression8,9.
NCBI Gene Bank ID
References & Citations
1. Zhou A, et al. (2005) J Interferon Cytokine Res. 25(10):595-603
2. Silverman RH. (2007) Cytokine Growth Factor Rev. 18(5-6):381-8
3. Wreschner DH, et al. (1981) Nucleic Acids Res. 9(7):1571-81
4. Cooper DA, et al. (2014) Nucleic Acids Res. 42(8):5202-16
5. Castelli JC, et al. (1997) J Exp Med. 186(6):967-72
6. Zhou A, et al (1997) EMBO J. 16(21):6355-63
7. Xiang Y, et al. (2003) Cancer Res. 63(20):6795-801
8. Meyer MS, et al. (2010) Carcinogenesis. 31(9):1597-603
9. Silverman RH. (2003) Biochemistry. 42(7):1805-12
Products are for research use only. Not for use in diagnostic or therapeutic procedures.