Caco-2 Whole Cell Lysate

Pricing & Details

Product No.C1182
Formats AvailableView All
Product Type
Cell Line Lysate
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C1182-0.1 mg
0.1 mg
$105.00
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Details

Cell Line
Caco-2 (Human Colorectal Adenocarcinoma)
Growth Media
ATCC Medium (Minimum Essential Medium (Eagle) with 2mM L-Glutamine, 1.5 g/L Sodium Bicarbonate, 0.1 mM non-Essential Amino Acids, 1.0 mM Sodium Pyruvate, and 20% FBS.)
Preparation
Caco-2 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Host
Human
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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