Donkey F(ab')2 Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) – DyLight® 550

Donkey F(ab')2 Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) – DyLight® 550

Product No.: R1304

[product_table name="All Top" skus="R1304"]

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Target
IgG (H&L)
Product Type
Secondary Antibody

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Product Size
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Antibody Details

Product Details

Reactive Species
Rat
Host Species
Donkey
Immunogen
Purified Rat IgG (H&L) (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This secondary antibody conjugate should be stored desiccated at 2-8°C.
Expiration Date
One year from date of receipt
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Excitation Laser
Yellow Laser (563 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Donkey F(ab')2 Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.