Goat F(ab’)2 Anti-Mouse IgG (H&L) (Adsorbed Against: Hu., Bv.) – HRP

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
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M103-0.5 ml
0.5 ml
$98.00
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Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified mouse IgG containing all isotypes.
Product Concentration
1.0 mg/ml
Formulation
This secondary antibody is formulated in 0.01M phosphate buffered saline (PBS) pH 7.4 containing 1.0% BSA and 50% glycerol. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This secondary antibody conjugate should be stored for up to 4 weeks at 2-8°C. However, for long term storage, aliquot into working volumes without dilution and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Expiration Date
One year from date of reconstitution
Country of Origin
USA
Applications and Recommended Usage
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Neutralization: The peptide may be used to neutralize the binding activity of PN:Z100 by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat F(ab')2 Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Protein
IgG (H&L)

References & Citations

  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.