Purified Rabbit IgG (>98%)
This secondary antibody is formulated in 0.05M Tris buffered saline pH 8.0 containing 0.001M MgCl<sub>2</sub>, 0.001M ZnCl<sub>2</sub>, 1.0% BSA, 50% glycerol and 0.09% sodium azide as a preservative.
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This secondary antibody conjugate should be stored for up to 4 weeks at 2-8°C. However, for long term storage, aliquot into working volumes without dilution and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
The suggested working dilution for immunohistology is approximately 1:150. However, each investigator should determine their own optimal working dilution for each specific research application
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat antibodies to rabbit IgG heavy and light chains are digested into F(ab')2 fragments using pepsin and then adsorbed against human serum proteins to minimize cross reactivity to human proteins.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.