Goat F(ab’)2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – APC

Purified Rabbit IgG (H+L) (>98%)

This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

One year from date of receipt.

Next Day 2-8°C

This secondary reagent has been optimally manufactured to detect primary rabbit antibodies. Leinco Technologies uses F(ab')₂ fragments to eliminate Fc receptor binding during use in immunohistochemistry, and flow cytometry. Leinco Technologies suggests using 1 microgram to stain 1 X 106 cells in flow cytometry. However, we suggest that each investigator determine their own optimal titer for other specific applications. High concentrations of glycerol such as contained in some mounting mediums will quench the fluorescent intensity of Allophycocyanin and other phycobilliproteins.

Red Laser (650 nm)

Goat F(ab')₂ Anti-Rabbit IgG (H&L) recognizes Rabbit IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.