Goat F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – APC

Goat F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – APC

Product No.: R128

[product_table name="All Top" skus="R128"]

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Target
IgG (H&L)
Product Type
Secondary Antibody

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Product Size
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Antibody Details

Product Details

Reactive Species
Rabbit
Host Species
Goat
Immunogen
Purified Rabbit IgG (H+L) (>98%)
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Expiration Date
One year from date of receipt.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This secondary reagent has been optimally manufactured to detect primary rabbit antibodies. Leinco Technologies uses F(ab')2 fragments to eliminate Fc receptor binding during use in immunohistochemistry, and flow cytometry. Leinco Technologies suggests using 1 microgram to stain 1 X 106 cells in flow cytometry. However, we suggest that each investigator determine their own optimal titer for other specific applications. High concentrations of glycerol such as contained in some mounting mediums will quench the fluorescent intensity of Allophycocyanin and other phycobilliproteins.
Excitation Laser
Red Laser (650 nm)
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat F(ab')2 Anti-Rabbit IgG (H&L) recognizes Rabbit IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.