Goat F(ab’)₂ Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu.) – Alk Phos

Purified Rat IgG (H&L) (>98%) containing all isotypes

This secondary antibody is supplied in 0.05M Tris pH 8.0, containing .001M MgCI2, .001M ZnCI2, 1.0% BSA and 0.09% sodium azide as a preservative.

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

This secondary antibody conjugate should be stored for up to 4 weeks at 2-8°C. However, for long term storage, aliquot into working volumes without dilution and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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The suggested working dilution for immunohistogy is approximately 1:150. However, each investigator should determine their own optimal working dilution for each specific research application.

Goat F(ab')₂ Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.