Applications and Benefits of Recombinant Mouse Thymus Chemokine-1

Recombinant Mouse Thymus Chemokine-1 (CXCL7) serves as a valuable tool for immunological research, particularly in studies examining thymic development and immune cell recruitment.

Immune System Development and Homeostasis

Chemokines like CXCL7 play a central role in the development and homeostasis of the immune system, being involved in both protective and destructive immune responses. This makes recombinant CXCL7 particularly useful for investigating the molecular mechanisms underlying T cell development and thymic function.

Thymocyte Development Research

CXCL7 is instrumental in chemokine-mediated choreography of thymocyte development. T cells develop in the thymus through interactions with stromal cells that deliver essential signals for thymocyte survival, proliferation, differentiation, and selection. By using recombinant CXCL7, researchers can experimentally manipulate these developmental pathways to better understand how chemokine signaling influences T cell maturation and selection processes.

Experimental Applications

The recombinant protein is suitable for multiple research applications:

• Cell culture studies: When formulated with carrier proteins like BSA, it is appropriate for use in cell or tissue culture experiments
• ELISA applications: It functions effectively as an ELISA standard or capture antibody in sandwich ELISA assays
• Cell biology research: It enables investigation of chemokine-mediated cellular responses and migration patterns

Research Advantages

Using recombinant CXCL7 allows researchers to conduct controlled, reproducible experiments with defined protein concentrations and purity. This standardization is essential for quantitative studies examining dose-dependent effects on immune cell behavior and thymic function, supporting investigations into both normal immune development and potential therapeutic interventions for immunodeficiency conditions.

Yes, recombinant mouse thymus chemokine-1 (CXCL7/TCK-1) is well-suited for use as a standard for quantification and calibration in ELISA assays.

Suitability as an ELISA Standard

Recombinant mouse CXCL7 is specifically designed and validated for this purpose. The recombinant protein is produced in E. coli and has been demonstrated to accurately quantitate both recombinant and natural forms of the chemokine. When natural mouse CXCL7 is measured against standard curves generated using the recombinant protein, the dose-response curves are parallel to those obtained with recombinant standards, indicating that the recombinant protein reliably represents the native molecule for quantification purposes.

Formulation Considerations

When selecting recombinant mouse CXCL7 for use as an ELISA standard, you should consider the formulation carefully. The protein is available in two primary formulations:

BSA-containing formulation — Recommended for use in cell or tissue culture applications and as an ELISA standard. The BSA carrier protein helps stabilize the recombinant protein during storage and handling.

Carrier-free formulation — Available as an alternative when carrier proteins may interfere with your specific assay requirements.

Molecular Specifications

The recombinant mouse CXCL7 standard typically spans amino acids 40-113 or 48-109, with a molecular weight of approximately 8.2 kDa. This corresponds to the mature, biologically active form of the chemokine suitable for quantitative applications.

Integration with Complete Assay Systems

Many complete ELISA kits include recombinant mouse CXCL7 as their calibration standard, with typical detection ranges of 10.97-8000 pg/mL and sensitivity values around 14 pg/mL. This demonstrates the protein's effectiveness across a wide dynamic range for quantification purposes.

Recombinant Mouse Thymus Chemokine-1 (also known as CXCL7) has been validated in published research primarily for the following applications:

• Chemotaxis assays: Recombinant Mouse Thymus Chemokine-1 has been used to induce and study chemotaxis (directed cell migration) in vitro, particularly in cell lines such as BaF3 mouse pro-B cells transfected with human CXCR2. This application is central to understanding the chemokine's role in leukocyte migration and immune cell trafficking.

• Cell culture studies: The recombinant protein is commonly used as a supplement in cell or tissue culture to investigate its biological effects on various cell types, including immune cells. This includes studies on cell signaling, proliferation, and migration.

• ELISA standard: Recombinant Mouse Thymus Chemokine-1 is frequently used as a standard in ELISA (enzyme-linked immunosorbent assay) to quantify endogenous levels of the chemokine in biological samples.

• Neutralization/Blocking studies: Antibodies against Mouse Thymus Chemokine-1 have been validated for their ability to neutralize the chemokine's activity in chemotaxis assays, demonstrating the specificity and functional relevance of the recombinant protein in these systems.

Additional context:

• The protein is also used in broader immunological research to study the regulation of leukocyte migration, activation, and the role of chemokines in inflammation and immune responses.
• While some sources mention its use in analytical testing and cell analysis, the most robust published validations are for chemotaxis, cell culture supplementation, and as an ELISA standard.

No evidence was found in the provided search results for in vivo validation or therapeutic studies specifically using recombinant Mouse Thymus Chemokine-1 (CXCL7) in animal models. Most published applications are in vitro or ex vivo.

Summary of validated applications:

• Chemotaxis assays (in vitro)
• Cell culture supplementation
• ELISA standard
• Neutralization/blocking studies

These applications are well-supported in the literature and by product validation data from research reagent providers.

Reconstitution Protocol

Recombinant Mouse Thymus Chemokine-1 (CXCL7, amino acids 48-109) should be reconstituted at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin (HSA or BSA). The protein is supplied as a lyophilized powder that has been filtered through a 0.2 μm membrane and formulated with BSA as a carrier protein.

Before opening the vial, briefly centrifuge it to bring the contents to the bottom. Add the appropriate volume of reconstitution buffer to achieve your desired concentration. Allow the reconstituted protein to sit at room temperature for several minutes to fully dissolve, ensuring complete hydration of the lyophilized material.

Storage Conditions

Short-term storage: After reconstitution under aseptic conditions, store the protein at 2–8°C for up to one month.

Long-term storage: For extended storage, maintain the protein at −20°C to −70°C in a manual defrost freezer. When storing lyophilized protein prior to reconstitution, keep it tightly sealed at −20°C.

Critical consideration: Avoid repeated freeze-thaw cycles, as these can compromise protein integrity and biological activity. If you require multiple aliquots, prepare them during initial reconstitution to minimize handling and freeze-thaw exposure.

Preparation for Cell Culture

When preparing the protein for cell culture experiments, maintain sterile conditions throughout the reconstitution process. Use sterile, distilled water or the specified PBS buffer system. The carrier protein (BSA or HSA) in the formulation helps stabilize the recombinant protein and prevents non-specific adsorption to container surfaces, which is particularly important for maintaining biological activity during cell culture applications.

For optimal results, prepare fresh working solutions from your stock immediately before use, and store any unused reconstituted material according to the storage guidelines above.