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Anti-Mouse CD11b (CD11b) Purified Low Endotoxin Functional Formulation

(Mac-1 αm chain)
Monoclonal Antibody

Prod. No.:C377
Isotype:Rat IgG2b
Conc.:0.5 mg/ml
Pkg. Size:0.5 mg, 1.0 mg, 5.0 mg, 25 mg, 100 mg
Starting at:$98.00
Availability:In Stock
Storage:Sterile 2-8°C Detailed storage instructions below.
Anti-Mouse CD11b (Mac-1) recognizes the (Mr 170 kD) αm subunit of Mac-1 (CD11b/CD18, αmβ2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).1

Antigen Distribution:
The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.

Known Species Cross Reactivity: Ms+
Host Species
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
>98% by SDS-PAGE and HPLC
<1.0 EU/µg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, IP, In vivo Depletion, B, FA
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 2.5 µg/ml.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD11b. A suggested method would be to stain cells expressing CD11b with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485).
Blocking: Please see referenced article2
Functional Assay: Activation of mouse peritoneal macrophages3
Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin
1. Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539
2. Springer, T.A., et al. (1982) Immunol. Rev. 68:171
3. Ding, A., et al. (1987) J Exp Med. 165:733
4. Vignali, D. A. et al. (1990) J. Immunol. 144:4030
5. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320
Related Products
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Anti-Human CD11b (Mac-1 Integrin αMβ2)-Fluorescein (FITC) (Leinco Prod. No.: C135)
Anti-Human CD11b (Mac-1 Integrin αMβ2) Purified (Leinco Prod. No.: C133)
Anti-Mouse CD11b (Mac-1 αm chain)-Biotin (Leinco Prod. No.: C228)
Anti-Mouse CD11b (Mac-1 αm chain)-Allophycocyanin (Leinco Prod. No.: C341)
Anti-Mouse CD11b (Mac-1 αm chain)-R-phycoerythrin (Leinco Prod. No.: C230)
Anti-Mouse CD11b (Mac-1 αm chain)-Fluorescein (Leinco Prod. No.: C229)
Anti-Mouse CD11b (Mac-1 αm chain) Purified (Leinco Prod. No.: C227)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-Allophycocyanin (APC) (Leinco Prod. No.: C307)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-R-phycoerythrin (R-PE) (Leinco Prod. No.: C136)
Anti-Human CD11b (Mac-1 Integrin αMβ2) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C368)

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