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Anti-Mouse CD11b (CD11b) Purified Low Endotoxin Functional Formulation

(Mac-1 αm chain)
Monoclonal Antibody


Prod. No.:C377
Clone:M1/70
Isotype:Rat IgG2b
Conc.:0.5 mg/ml
Pkg. Size:0.5 mg, 1.0 mg, 5.0 mg, 25 mg, 100 mg
Starting at:$98.00
Availability:In Stock
Storage:Sterile 2-8°C Detailed storage instructions below.
Description
Specificity:
Anti-Mouse CD11b (Mac-1) recognizes the (Mr 170 kD) αm subunit of Mac-1 (CD11b/CD18, αmβ2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).1

Antigen Distribution:
The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity
>98% by SDS-PAGE and HPLC
Endotoxin
<1.0 EU/µg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC, IP, In vivo Depletion, B, FA
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 2.5 µg/ml.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD11b. A suggested method would be to stain cells expressing CD11b with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485).
Blocking: Please see referenced article2
Functional Assay: Activation of mouse peritoneal macrophages3
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539
2. Springer, T.A., et al. (1982) Immunol. Rev. 68:171
3. Ding, A., et al. (1987) J Exp Med. 165:733
4. Vignali, D. A. et al. (1990) J. Immunol. 144:4030
5. Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320
Related Products
Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485)
Anti-Human CD11b (Mac-1 Integrin αMβ2) Purified (Leinco Prod. No.: C133)
Anti-Human CD11b (Mac-1 Integrin αMβ2) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C368)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-R-phycoerythrin (R-PE) (Leinco Prod. No.: C136)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-Fluorescein (FITC) (Leinco Prod. No.: C135)
Anti-Mouse CD11b (Mac-1 αm chain)-Biotin (Leinco Prod. No.: C228)
Anti-Mouse CD11b (Mac-1 αm chain)-Allophycocyanin (Leinco Prod. No.: C341)
Anti-Mouse CD11b (Mac-1 αm chain)-R-phycoerythrin (Leinco Prod. No.: C230)
PN:
Anti-Mouse CD11b (Mac-1 αm chain)-Fluorescein (Leinco Prod. No.: C229)
Anti-Mouse CD11b (Mac-1 αm chain) Purified (Leinco Prod. No.: C227)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-Biotin (Leinco Prod. No.: C134)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-Allophycocyanin (APC) (Leinco Prod. No.: C307)

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