Product Datasheet
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Goat Anti-Mouse IgG (H&L)

AMCA
Secondary Antibody
Adsorbed Against: Hu., Rt., Rb., Bv., Hs.

Size


Prod. No.:M392
Conc.:1.4 mg/ml
Pkg. Size:0.5 mg
Starting at:$170.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Goat Whole Molecule Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
AMCA is a small fluorescent organic molecule with an absorbance maximum of 350 nm and an emission maximum of 450 nm.

Host Species
Goat
Immunogen
Purified Mouse IgG (H&L) (>98%)
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Reconstitution
The lyophilized secondary antibody AMCA conjugate should be reconstituted in 0.4 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates. After reconstitution, add an equal volume of ACS grade glycerol bringing the final concentration to 50% v/v.
Expiration Date
One year from date of reconstitution
Storage and Stability
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted AMCA conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily.

IHC: 1:50 - 1:200
FC: 1:50 - 1:200
IF: 1:50 - 1:200

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Country of Origin
USA
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