Anti-Calnexin (NT) Serum
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Antibody DetailsProduct DetailsReactive Species Bovine ⋅ Canine ⋅ Chicken ⋅ Guinea Pig ⋅ Hamster ⋅ Human ⋅ Monkey ⋅ Mouse ⋅ Pig ⋅ Rabbit ⋅ Rat ⋅ Sheep Host Species Rabbit Immunogen A 19 amino acid synthetic peptide corresponding to canine calnexin conjugated to KLH. Product Concentration Lot Specific Formulation Whole antiserum State of Matter Liquid Product Preparation Whole antiserum Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~90 kDa is detected.
These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rabbit Polyclonal Antibody specific to Calnexin Background Calnexin is a ~90 kDa integral protein of the endoplasmic reticulum; it is also referred to as IP90, p88 and p90. It consists of a 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix, and a short acidic cytoplasmic tail. Its positively charged cytosolic residues ensure its retention in the endoplasmic reticulum. Calnexin together with calreticulin play a key role in glycoprotein folding by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor, and B cell surface immuno- globulin. Function Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical Protocols Certificate of Analysis |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
