Anti-Hsp40 (Hdj1) Antibody (11111)

Anti-Hsp40 (Hdj1) Antibody (11111)

Product No.: 11111


Clone 3B9.E6 Target Hsp40 (Hdj1) Formats AvailableView All Product Type Monoclonal Alternate Names DnaJ protein homolog 1, Heat shock 40 kDa protein 1, HSP40, Heat shock protein 40, Human DnaJ protein 1, hDj-1 Isotype Mouse IgG1 Applications ELISA , IHC , IP , WB


Select Product Size 100 µg — $384.00	Qty Max: Min: 1 Step: 1 $384.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Antibody Details Product Details Reactive Species Human/Mouse/Rat Host Species Mouse Immunogen Recombinant Hsp40 (Hdj1) Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected.ELISA User should determine optimal concentrations for their application. Positive control: HeLa cell lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity This antibody recognizes human, mouse, and rat Hsp40 (Hdj1). Other species have not been tested. It does not cross-react with Hdj2 or Ydj1. Background Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins. Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}. NCBI Gene Bank ID DNAJB1 UniProt.org P25685 Research Area Heat Shock & Stress Proteins References & Citations View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human/Mouse/Rat

Host Species

Mouse

Immunogen

Recombinant Hsp40 (Hdj1)

Product Concentration

Lot Specific

Formulation

This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.

State of Matter

Liquid

Product Preparation

Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected.ELISA

User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human, mouse, and rat Hsp40 (Hdj1). Other species have not been tested. It does not cross-react with Hdj2 or Ydj1.

Background

Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins.

Function

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}.

NCBI Gene Bank ID

DNAJB1

UniProt.org

P25685

Research Area

Heat Shock & Stress Proteins