Anti-Hsp40 (Ydj1) Antibody (11114)

Anti-Hsp40 (Ydj1) Antibody (11114)

Product No.: 11114

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Clone
1G10.H8
Target
Hsp40 (Ydj1)
Formats AvailableView All
Product Type
Monoclonal
Alternate Names
Yeast dnaJ protein 1
Isotype
Mouse IgG1
Applications
ELISA
,
IP
,
WB

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Antibody Details

Product Details

Reactive Species
Yeast
Host Species
Mouse
Immunogen
Full-length yeast Hsp40 (Ydj1)
Product Concentration
Lot Specific
Formulation
This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added.
State of Matter
Liquid
Product Preparation
Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at 0.5-1ug/ml. A band of ~40 kDa is detected

User should should determine optimal concentrations for their application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This antibody recognizes yeast Hsp40 (Ydj1). It does not cross-react with human, mouse, or rat Hsp40.
Background
Members of the human Hsp40/DnaJ protein family feature a J domain (named for the bacterial DnaJ protein). The J domain spans the first 75 N- terminal amino acids and is separated from the C-terminus by a glycine- phenylalanine-rech domain. The Hsp40/DnaJ proteins play many different roles in cellular processes such as folding, translocation, degradation, and assembly of multi-protein complexes. Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding as well as in regulation of Hsp70 function. Hsp40 stimulates ATPase activity of Hsp70 which in turn causes conformational changes in unfolded proteins.
Function
Probably involved in mitochondrial protein import. Is also required for efficient translocation of pre-pro-alpha-factor. Involved in heme regulation of HAP1, as a component of the high-molecular-weight (HMC) complex. {PubMed:11689685}.
NCBI Gene Bank ID
UniProt.org
Research Area
Heat Shock & Stress Proteins

References & Citations

Indirect Elisa Protocol
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
11114
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.