Anti-Human BCL-2 (124)- APC

Anti-Human BCL-2 (124)- APC

Product No.: B122

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Clone
124
Target
Bcl-2
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse IgG1 κ
Applications
ELISA
,
FC
,
IF
,
IF Staining
,
IHC
,
IHC FFPE
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Immunogen
Peptide sequence corresponding to residues 41-54 of Human BCL-2
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Excitation Laser
Red Laser (650 nm)
Additional Applications Reported In Literature ?
ELISA
WB
IHC
FC
IP
IF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 124 activity is directed against human BCL-2
Background
BCL-2 is a member of a protein family that regulates apoptosis, with some members inducing (proapoptotic) and others inhibiting (antiapoptotic) apoptosis 1. BCL-2 acts as an antiapoptotic protein that prevents apoptosis and promotes survival in response to many cell stresses and cytotoxic chemicals 2 via the mitochondria-mediated intrinsic apoptosis pathway 1. BCL-2 is also an important oncogene whose overexpression is common in hematological malignancies such as follicular lymphoma, chronic lymphocytic leukemia, mantle cell leukemia and Waldenström's macroglobulinemia 1 as well as breast cancer 3. As such, BCL-2 is a target for inhibition in cancer immunotherapy. Additionally, BCL-2 is involved in cardiac hypertrophy and has been suggested as a potential therapeutic target for clinical management 4.

Clone 124 was generated by immunizing mice with a synthetic peptide corresponding to amino acids 41 to 54 (GAAPAPGIFSSQPG) of BCL-2 conjugated to thyroglobulin 5. Sera were screened on cryostat sections of human tonsil and on a follicular lymphoma with a known 14;18 translocation. Cell fusion and hybridoma lines were produced by conventional technology. 124 recognizes BCL-2 by Western blotting under both reducing and non-reducing conditions.
Antigen Distribution
BCL-2 is a mitochondrial membrane protein, also found in the nuclear envelope and to a lesser degree in the cell membrane, that is detected in lymphocytes, T cells, B cells, primary lymphoid follicles, the mantle zone of secondary follicles, non-lymphoid hematopoietic precursors such as myeloid cells, exocrine gland duct cells, uterine smooth muscle, endometrial glands, thyroid follicles, prostatic glands, breast lobules, fetal epithelial germinative regions, placental trophoblast, endocrine cells and neurons as well as follicular non-Hodgkin’s lymphomas and a variety of other B cell lymphomas and epithelial neoplasms.
Ligand/Receptor
BAD, BAK proteins
NCBI Gene Bank ID
UniProt.org
Research Area
Biomarker
.
Cancer
.
Immunology

References & Citations

1. Xu Y, Ye H. Exp Hematol Oncol. 11(1):31. 2022.
2. Papadimitriou CS, Costopoulos JS, Christoforidou BP, et al. Eur J Cancer. 33(8):1275-1280. 1997.
3. Merino D, Lok SW, Visvader JE, et al. Oncogene. 35(15):1877-1887. 2016.
4. Meng X, Cui J, He G. Biomed Res Int. 2021:6615502. 2021.
5. Pezzella F, Tse AG, Cordell JL, et al. Am J Pathol. 137(2):225-232. 1990.
6. Monaghan P, Robertson D, Amos TA, et al. J Histochem Cytochem. 40(12):1819-1825. 1992.
7. Lu QL, Hanby AM, Nasser Hajibagheri MA, et al. J Cell Sci. 107 (Pt 2):363-371. 1994.
8. Willingham MC, Bhalla K. J Histochem Cytochem. 42(4):441-450. 1994.
9. Schandl CA, Li S, Re GG, et al. J Histochem Cytochem. 47(2):151-158. 1999.
Indirect Elisa Protocol
Flow Cytometry
IF
IF Staining
IHC
IHC FFPE
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.