Anti-Human CCL1 (Clone 35305)

Product No.: I-643


Clone 35305 Target CCL1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYA1, I-309, T Cell Activation Gene 3, P500, SISe Isotype IgG1 Applications ELISA Cap , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CCL1 (Accession # P22362) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830765 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-177 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-224. Western Blotting: To detect Human CCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL1 is 1.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-224. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human I-309 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human I-309 (at 0.02 ng/ml) is 0.1 - 0.5 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human C-C motif chemokine 1 (CCL1) (Clone 35305) recognizes an epitope on Human CCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.¹ CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.² CCL1 may play a role in lymphocyte recruitment in bronchial asthma.³ PubMed CCL1 Research Area Chemokine References & Citations 1. Krangel, MS. et al. (1992) Proceedings of the National Acad of Sci. 89: 2950 2. Roos, RS. et al. (1997) J Biol Chem. 272: 17251 3. Teran, LM et al. (2006) Eur Respir J. 28: 59 View product citations for antibody I-643 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CCL1 (Accession # P22362)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830765

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-177 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-224.
Western Blotting: To detect Human CCL1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL1 is 1.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-224.

Additional Applications Reported In Literature ?

Neutralization: This antibody is useful for neutralization of Human I-309 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human I-309 (at 0.02 ng/ml) is 0.1 - 0.5 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human C-C motif chemokine 1 (CCL1) (Clone 35305) recognizes an epitope on Human CCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.¹ CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.² CCL1 may play a role in lymphocyte recruitment in bronchial asthma.³

PubMed

CCL1

Research Area

Chemokine

References & Citations

1. Krangel, MS. et al. (1992) Proceedings of the National Acad of Sci. 89: 2950
2. Roos, RS. et al. (1997) J Biol Chem. 272: 17251
3. Teran, LM et al. (2006) Eur Respir J. 28: 59

View product citations for antibody I-643 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
I-643	Anti-Human CCL1 (Clone 35305)
T458	Anti-Mouse CCL1 (Clone 148113)
I-231	Anti-Human CCL1
C1483	Anti-Mouse CCL1
T459	Anti-Mouse CCL1 (Clone 148107)
I-224	Recombinant Human CCL1
T410	Recombinant Mouse CCL1
T504	Anti-Mouse CCL1-Biotin

Formats Available

Prod No.	Description
I-643	Anti-Human CCL1 (Clone 35305)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human CCL1 (Clone 35305)