Anti-Human CD16 (Clone 3G8) – DyLight® 650

Anti-Human CD16 (Clone 3G8) – DyLight® 650

Product No.: C6857

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Clone
3G8
Target
CD16
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
FcγRIII, Fc Gamma RIII
Isotype
IgG1 κ
Applications
FC

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Antibody Details

Product Details

Reactive Species
Baboon
Chimpanzee
Cynomolgus Monkey
Marmoset
Pigtailed Macaque
Rhesus Monkey
Squirrel Monkey
Human
Host Species
Mouse
Immunogen
Human PMN cells
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (652 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 3G8 antibody for staining cells in flow cytometry is ≤ 5.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
B
CyTOF®
CODEX
IHC (Frozen)
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.
Background
CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.
Antigen Distribution
CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
NCBI Gene Bank ID
Research Area
Immunology
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Innate Immunity

References & Citations

1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497
2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.