Anti-Human CD16 (Clone 3G8) – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2858
Clone
3G8
Protein
CD16
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
FcγRIII, Fc Gamma RIII
Isotype
Mouse
IgG1 κ
Applications
B
,
CyTOF®
,
FC
,
IHC FF
,
IP
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2858-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C2858-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C2858-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
C2858-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
C2858-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Baboon
Chimpanzee
Cynomolgus Monkey
Marmoset
Pigtailed Macaque
Rhesus Monkey
Squirrel Monkey
Human
Host Species
Mouse
Immunogen
Human PMN cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 3G8 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
CyTOF®
IHC (Frozen)
IP
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.
Antigen Distribution
CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.
Background
CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes1. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils1. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)2.

Antigen Details

Protein
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology

References & Citations

1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497
2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47
Flow Cytometry
IHC
Immunoprecipitation Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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