Anti-Human CD16 [3G8] – Purified in vivo PLATINUM™ Functional Grade

Mouse IgG1 PLATINUM, Clone hksp OR Mouse IgG1 PLATINUM, Clone hksp84

Human PMN cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

CyTOF®
IHC (Frozen)
IP
B

Clone 3G8 recognizes an epitope on human CD16. This clone also cross-reacts with non-human primate CD16.

CD16 antibody, 3G8, recognizes human CD16, also known as low-affinity IgG receptor III (FcγRIII). There are two distinct forms of CD16, CD16a (FcγRIIIa) and CD16b (FcγRIIIb). CD16a is a 50-65 kDa heterooligomeric polypeptide-anchored transmembrane protein expressed by NK cells, macrophages, and subsets of monocytes¹. CD16b is a 48 kDa monomeric glycosylphosphatidylinositol (GPI)-anchored protein expressed on neutrophils¹. Upon binding to the Fc portion of IgG or IgG-antigen complex, both CD16 isoforms induce signaling cascades resulting in multiple functions, including phagocytosis, cytokine release, proliferation, degranulation, and antibody dependent cell-mediated cytotoxicity (ADCC)².

CD16 is expressed on NK cells, activated monocytes, macrophages, neutrophils, and placental trophoblasts.

Aggregated IgG, IgG-antigen complex

Low affinity IgG Fc receptor, phagocytosis, ADCC

1. Ravetch JV & Perussia B. (1989) J Exp Med. 170(2):481-497
2. Nimmerjahn F & Ravetch JV (2008) Nat Rev Immunol. 8(1):34-47