Anti-Human CD4 (Clone OKT-4) – Purified in vivo PLATINUM™ Functional Grade
Anti-Human CD4 (Clone OKT-4) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C9205
Clone OKT-4 Target CD4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD4; T4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 Isotype Mouse IgG2b κ Applications ELISA , FC , ICC , IF Staining , IHC FF , IHC FFPE , in vivo , IP , N , PhenoCycler® , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human peripheral T cells Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829880 Applications and Recommended Usage? Quality Tested by Leinco ELISA FC WB Additional Applications Reported In Literature ? PhenoCycler-Fusion (CODEX)® IF Staining IP IHC FF IHC FFPE ICC N Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other. Background CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Antigen Distribution CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. PubMed NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The monoclonal antibody clone OKT-4 is used in in vivo mouse studies primarily to target and deplete human CD4+ T cells in humanized mouse modelsmice that express human CD4, often replacing the murine CD4 gene. This specificity means OKT-4 does not bind mouse CD4 but is valuable for functional studies of the human immune system in a mouse setting. Essential context and supporting details:
Summary Table:
According to supplier and research literature, the key role of OKT-4 in in vivo mouse studies is for selective manipulation of human CD4+ T cells in mouse models with a humanized immune system, providing a powerful tool for immunology and translational research. Commonly used antibodies or proteins alongside OKT-4 (an anti-human CD4 antibody) in the immunology literature include other T cell markers, costimulatory molecules, and leukocyte subset markers. These combinations are typically used for detailed phenotyping of immune cell subsets and functional studies. Frequently combined antibodies or proteins with OKT-4:
Functional protein combinations:
Rationale:OKT-4 specifically binds the CD4 molecule, present on most helper T cells and subsets of monocytes/macrophages, facilitating fine phenotypic and functional characterization of these cells. Other antibodies are selected based on the specific research focus (e.g., co-stimulatory status, exclusion of non-T-cell populations, identification of regulatory T cell subsets). Notes:
In summary, CD3, CD8, CD45, CD19/20, CD14, and activation/costimulatory markers are most commonly used with OKT-4 in experimental immunology to comprehensively profile T cells and other immune subsets. Key findings from clone OKT-4 (often cited as OKT4 or OKT4+) in scientific literature primarily relate to two distinct contexts: immunology (particularly characterization of T helper cells using the OKT4 monoclonal antibody) and developmental biology (studies of Oct4 gene expression in cloning and pluripotency). If your query refers to the OKT4 monoclonal antibody (commonly used for human CD4 cell studies), the main findings are as follows:
Key immunological findings from OKT4 citations:
If your question refers to Oct4 in developmental biology (sometimes confusingly similar in search results), key findings relate to:
If you intended a particular context, specifying "OKT4 antibody clone" (immunology) or "Oct4 gene in clones" (developmental biology/pluripotency) can refine the answer further. Dosing regimens for OKT-4 (anti-CD4, clone OKT4) vary based on the specific mouse model, disease context, and study aim, and information must often be adapted from studies using analogous anti-CD4 antibodies, as OKT-4 is a well-established anti-human CD4 clone but is less commonly used in mice compared to clones like GK1.5. Key findings from available sources:
For comparison, in common in-vivo mouse-specific anti-CD4 antibodies (example: GK1.5), the standard dosing for murine CD4 depletion is typically 200–250 µg per mouse, given 2–3 times per week by intraperitoneal injection, mainly in CD4+ T cell depletion studies in standard immunology mouse models. These doses are much lower than humanized antibody or OKT-4 doses because of differences in affinity, isotype, and target abundance. Summary Table: OKT-4 Dosing in Mouse Models
Critical distinctions:
No evidence was found of standardized OKT-4 regimens in non-transgenic mice, and protocol variability is considerable depending on the experimental aims. Where not specified, check the latest literature for your specific mouse model and experimental goal, as optimal regimens may differ. References & Citations1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66. 2. Ploss, A. et al. (2018) Nat Commun 9(1):5031 3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754. Technical ProtocolsCertificate of Analysis |
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