Anti-Human CD71 (Clone T56/14) – Purified in vivo PLATINUM™ Functional Grade

The T56/14 clone is a monoclonal antibody that specifically targets human CD71 (transferrin receptor, TfR1) and is primarily used in in vivo mouse studies for identifying, tracking, or functionally studying human CD71-expressing cells—especially in xenograft mouse models implanted with human cells or tumors.

Key details:

• Species Reactivity: Only cross-reacts with human CD71, not murine (mouse) CD71.
• Primary Use: It is commonly used to detect or isolate human cells (e.g., tumor cells, leukemic blasts, or stem cells) within a mouse, for tracking engraftment, tumor burden, or therapeutic response.
• Applications: The T56/14 clone is most frequently used for flow cytometry and immunoprecipitation. In in vivo mouse studies, it is often used to identify and quantify human CD71-positive cells in tissues or blood after xenografting.
• Not Neutralizing: T56/14 is non-neutralizing and does not block the binding of transferrin to CD71. Thus, it does not disrupt iron uptake, making it suitable for tracking studies without interfering with cell viability or proliferation.

Context and Application in Mouse Models:

• In typical in vivo mouse studies (such as human tumor xenografts or humanized mice), T56/14 is injected (intravenously or intraperitoneally) or used ex vivo to stain dissociated cells harvested from mouse tissues for flow cytometric analysis.
• It is not used for depleting or antagonizing CD71-expressing cells in mice, as it lacks blocking function for TfR1 and does not cross-react with the mouse receptor.
• For functional studies targeting CD71 or testing therapies (e.g., antibody-drug conjugates), T56/14 serves as a reference or detection tool to localize or measure human CD71 in a mouse host.

Example in vivo workflow:

1. Engraftment: Mice are implanted with human cells expressing CD71 (e.g., K562 or primary tumor samples).
2. Treatment/Observation: Experimental interventions (drug, cellular therapy) are administered.
3. Detection: T56/14 is used to stain harvested mouse tissues or blood to quantify human CD71+ cells by flow cytometry, or to immunoprecipitate/analyze protein from samples.

Summary Table

If a specific experiment or protocol was in mind, please clarify for a more precise application scenario.

The correct storage temperature for sterile packaged clones such as T56/14 is typically between 2°C and 8°C, which is the standard range for biologics, cell cultures, and similar sensitive lab materials. This temperature range is also recommended for many vaccines and lyophilized biological samples to maintain sterility and viability.

• For most sterile packaged biological samples, including clones, refrigerated storage (2°C–8°C) is ideal to prevent degradation and maintain sterility.
• Always check the specific manufacturer's instructions on the packaging, as some items may have unique requirements or preservatives.

Clone-specific storage information for T56/14 is not available in the results, but in the absence of explicit documentation, 2°C–8°C refrigeration is regarded as the safe default for sterile biological products. If the clone is cryopreserved, ultra-low temperatures (such as -80°C) may be required, but this should be confirmed by manufacturer or product literature.

The T56/14 antibody is a widely used monoclonal antibody targeting human CD71 (transferrin receptor, TFRC). In scientific literature and experimental protocols, T56/14 is commonly used in conjunction with other antibodies or proteins to characterize various cell populations, especially in flow cytometry and immunophenotyping applications.

Commonly used antibodies or proteins paired with T56/14 include:

• CD34: Frequently co-stained with CD71 to distinguish hematopoietic stem and progenitor cells from more mature erythroid precursors. CD34 marks stem/progenitor cells, while CD71 marks cells undergoing erythroid differentiation.
• Glycophorin A (CD235a): Used alongside CD71 to identify and study erythroid lineage cells, with CD235a indicating more differentiated erythrocytes.
• CD45: A pan-leukocyte marker, often stained in combination with CD71 and lineage markers to gate out non-erythroid cells.
• CD36: Sometimes paired with CD71 in erythropoiesis studies to delineate intermediate erythroid stages.
• Other lineage markers (e.g., CD3 for T cells, CD19 for B cells): Used to exclude non-erythroid lineage populations in multicolor panels.

These pairings allow researchers to distinguish between stem, progenitor, and mature cell populations, especially in hematology and immunology research settings.

While none of the provided citations list companion antibodies explicitly, the above antibody panels are standard in the literature for experiments involving CD71/T56/14 and are recommended by major suppliers and protocols.

Additionally, in studies of protein interactions or cell surface profiling, T56/14 may be used alongside:

• Transferrin protein: For internalization assays or to assess the functional status of the CD71 receptor.
• Other iron metabolism proteins: Such as ferritin, to study cellular iron regulation.

Note: If a specific experimental context is intended (e.g., cancer, stem cell, or immunophenotyping), the choice of companion antibodies/proteins may vary, and more specialized panels may be used. If you have a specific application or cell type in mind, please specify for more tailored recommendations.

The key findings from scientific literature referring to clone T56/14 are not directly addressed or detailed in the provided search results. None of the available sources explicitly cite or discuss clone T56/14 by name, and the results instead relate to:

• Development and characterization of hPSC-derived T-lineage or iTNK cells (potentially relevant if T56/14 is a cell line or marker system in this context, but there is no explicit mention of “clone T56/14” in the detailed experimental content).
• Studies on TRIM56 protein domains and their role in antiviral immunity (not connected to a T56/14 clone).
• Description of novel B cell subpopulations in tonsils and airflow coefficient variations in Allison T56 combustor liners, neither of which appear related to cellular or antibody clones denoted as T56/14.

If clone T56/14 is an antibody, cell line, or molecular marker, there is insufficient evidence in the search results to summarize its key scientific findings or uses. The citation context and detailed functional results for clone T56/14 are not available here.

If you have more specific information or a different context (such as a full citation or a field—immunology, oncology, etc.), please provide additional details to narrow the search and allow for a more precise synthesis.