Anti-Human CLC (Clone 138815)

Product No.: C1462


Clone 138815 Target CLC Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Cardiotrophin-Like Cytokine, NR6, B Cell Stimulating Factor-3 (BSF3), Novel Neurotrophin-1 (NNT1), CISS2, CLCF1 Isotype IgG2b Applications ELISA Indirect , IHC , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen E. coli-Derived Recombinant Human CLC (Accession # Q9UBD9) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828526 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human CLC is approximately 5 ng/well. Western Blotting: To detect Human CLC this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CLC is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Cardiotrophin-Like Cytokine (CLC) (Clone 138815) recognizes an epitope on Human CLC. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.¹ CLC is expressed highly in spleen and peripheral leukocytes² and is a target-derived factor required for the survival of specific pools of motoneurons.³ CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.⁴ PubMed CLC NCBI Gene Bank ID 23529 UniProt.org More Information on Uniprot.org References & Citations 1. Taga, T. et al. (2002) Cytokine 18: 1 2. Shi, Y. et al. (1999) Biochemical Biophysical Res Communications 262: 132 3. Oppenheim, RW. et al. (2003) J Neurosci. 23: 8854 4. Wen, R. et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036 View product citations for antibody C1462 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

E. coli-Derived Recombinant Human CLC (Accession # Q9UBD9)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828526

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human CLC is approximately 5 ng/well.
Western Blotting: To detect Human CLC this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CLC is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

Immunohistochemistry: Suitable for use at concentration of 8-25 µg/mL.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Cardiotrophin-Like Cytokine (CLC) (Clone 138815) recognizes an epitope on Human CLC. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Cardiotrophin-like cytokine (CLC) also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3) is a member of the interleukin (IL)-6 family of cytokines.¹ CLC is expressed highly in spleen and peripheral leukocytes² and is a target-derived factor required for the survival of specific pools of motoneurons.³ CLC shows neuroprotective effects on retinal ganglion cells (RGCs) in vivo and might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.⁴

PubMed

CLC

NCBI Gene Bank ID

23529

UniProt.org

More Information on Uniprot.org

References & Citations

1. Taga, T. et al. (2002) Cytokine 18: 1
2. Shi, Y. et al. (1999) Biochemical Biophysical Res Communications 262: 132
3. Oppenheim, RW. et al. (2003) J Neurosci. 23: 8854
4. Wen, R. et al. (2005) Graefe's Archive for Clinical and Experimental Ophthalmol. 243: 1036

View product citations for antibody C1462 on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
N147	Anti-Human CLC
C1462	Anti-Human CLC (Clone 138815)
N148	Anti-Human CLC-Biotin

Formats Available

Prod No.	Description
C1462	Anti-Human CLC (Clone 138815)

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Anti-Human CLC (Clone 138815)