Anti-Human MIP-3β (Clone 54909)

Pricing & Details

Product No.M1094
Clone
54909
Protein
MIP-3 beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL19, AMAC-1, Ckb7, Ck-Beta 7, DC-CK1, MIP4, PARC, SCYA18, MIP-3B, Exodus-3, EBI1-Ligand Chemokine (ELC)
Isotype
Mouse
IgG2b
Applications
ELISA Indirect
,
FC
,
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1094-25 µg
25 µg
$195.00
In stock
Max:
Min: 1
Step: 1
M1094-100 µg
100 µg
$430.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MIP-3β (Accession # Q99731.1)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-3β. A suggested method would be to stain cells expressing MIP-3β with 0.25 µg per 1-5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189.
Western Blotting: To detect Human MIP-3β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-3β is 100 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M168.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Macrophage Inflammatory Protein-3 Beta (MIP-3β) (Clone 54909) recognizes an epitope on Human MIP-3β. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Macrophage inflammatory protein-3beta (MIP-3beta) is a non-glycosylated polypeptide and member of the B-chemokine (C-C) family of cytokines. It plays an important role in the inflammatory response of human T cells and tissue macrophages. MIP-3beta is a potential chemoattractant for endometrial natural killer cells.1 It may be important in trafficking of T cells in thymus, and T cell and B cell migration to secondary lymphoid organs.2

Antigen Details

Protein

References & Citations

1. Honjo, H. et al. (2004) Fertil Steril. 1: 876
2. Broxmeyer, HE. et al. (1998) J Immunol. 160: 2418
Indirect Elisa Protocol
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Prod No.
Description
M1094
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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