Anti-Human MIP-3β (Clone 54909)
Anti-Human MIP-3β (Clone 54909)
Product No.: M1094
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Clone 54909 Target MIP-3 beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL19, AMAC-1, Ckb7, Ck-Beta 7, DC-CK1, MIP4, PARC, SCYA18, MIP-3B, Exodus-3, EBI1-Ligand Chemokine (ELC) Isotype IgG2b Applications ELISA Indirect , FC , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human MIP-3β (Accession # Q99731.1) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831226 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-3β. A suggested method would be to stain cells expressing MIP-3β with 0.25 µg per 1-5 x 106 cells in a total staining volume of ≤ 200 µl followed by PN:M1189. Western Blotting: To detect Human MIP-3β this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-3β is 100 and 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M168. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Macrophage Inflammatory Protein-3 Beta (MIP-3β) (Clone 54909) recognizes an epitope on Human MIP-3β. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Macrophage inflammatory protein-3beta (MIP-3beta) is a non-glycosylated polypeptide and member of the B-chemokine (C-C) family of cytokines. It plays an important role in the inflammatory response of human T cells and tissue macrophages. MIP-3beta is a potential chemoattractant for endometrial natural killer cells.1 It may be important in trafficking of T cells in thymus, and T cell and B cell migration to secondary lymphoid organs.2 Antigen DetailsPubMed References & Citations1. Honjo, H. et al. (2004) Fertil Steril. 1: 876 2. Broxmeyer, HE. et al. (1998) J Immunol. 160: 2418 Technical Protocols  IHC FFPE  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
