Anti-Human/Monkey CD40L (CD154) [Clone 5C8] — Recombinant in vivo Functional Grade
Anti-Human/Monkey CD40L (CD154) [Clone 5C8] — Recombinant in vivo Functional Grade
Product No.: C585
Data
Western Blot
A 5.0ug sample of Human CD40L was loaded onto an SDS-PAGE gel under reducing conditions and probed with 5C8 (Leinco Prod. No.: C585) @ 10ug/mL, Goat Anti-Mouse IgG secondary antibody 1:5000 dilution (Goat Anti-Mouse IgG (H+L) adsorbed against ms HRP Leinco Prod. No.: M114)
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Monkey Host Species Mouse Immunogen Human D1.1 T cell line Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% by SDS Page ⋅ ≥95% monomer by analytical SEC Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. State of Matter Liquid Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? FA, FC, IP B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 5C8 is a mouse monoclonal antibody that targets the human and cynomolgus monkey
CD154 (CD40L).
Background CD154, also known as CD40L, gp39, TRAP, or T BAM is a 39 kDa molecule that plays a vital role in the immune system. It belongs to the tumor necrosis factor (TNF) family and is crucial for the interaction between antigen-specific lymphocytes and antigen-presenting cells1,2. By binding to CD40 it assists in the costimulation process required for activating T cells and promoting B cell proliferation. CD40L also stimulates the secretion of immunoglobulin and facilitates Ig isotype switching when it binds to CD40 on B-cells3. Moreover, CD40L regulates cytokine production by T cells4. Due to its involvement in immune responses, this molecule is of significant importance for immunological research and therapeutic interventions5,6. Clone 5C8 is a mouse monoclonal antibody produced through immunization of BALB/c mice with cells from a mouse myeloma cell line. It specifically recognizes human CD154 and binds to an epitope that differs from the CD40 binding site7,8. Antigen Distribution CD154 is primarily expressed on activated T-cells but can also be found in other cell
types, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, smooth muscle cells, endothelial cells, and epithelial cells.
Ligand/Receptor CD40 NCBI Gene Bank ID UniProt.org Research Area Biomarker . Cancer . Immunology . Organoid References & Citations1. Seijkens T, Engel D, Tjwa M, Lutgens E. Thromb Haemost. 104(4):693-701. 2010. 2. Mackey MF, Barth RJ, Noelle RJ. J Leukoc Biol. 63(4):418-428. 1998. 3. Aversa G, Punnonen J, Carballido JM, Cocks BG, De Vries JE. Seminars in Immunology. 6(5):295-301.1994. 4. Armitage RJ, Tough TW, Macduff BM, et al. Eur J Immunol. 23(9):2326-2331.1993. 5. Jabara HH, Brodeur SR, Geha RS. J Clin Invest. 107(3):371-378. 2001. 6. Singh J, Garber E, Van Vlijmen H, et al. Protein Sci. 7(5):1124-1135. 1998. 7. Salvadori B, Cusumano F, Del Bo R, et al. Cancer. 63(12):2532-2536. 1989. 8. Alturaihi H, Hassan GS, Al-Zoobi L, et al. Eur J Immunol. 45(2):592-602. 2015. 9. Nadazdin O, Boskovic S, Wee SL, et al. The Journal of Immunology. 187(9):4589-4597. 2011. Technical ProtocolsB FA   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
