Anti-Human SMAC

E. coli-Derived Recombinant Human SMAC/Diablo

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Lyophilized

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Next Day Ambient

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is PN:R1215. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Rat Anti-Human Second Mitochondria Derived Activator of Caspase (SMAC) recognizes Human SMAC. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Second Mitochondria Derived Activator of Caspase (SMAC) is a mitochondrial apoptogenic protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs.¹ SMAC promotes cytochrome-c dependent activation by eliminating the inhibition of IAP - a protein that negatively regulates apoptosis or programmed cell death.²

1. Melendez-Zajgla, J. et al. (2008) J Exp Clin Cancer Res. 26: 27
2. Vucic, D. et al. (2002) J Biol chem. 277: 12275