Anti-Mouse CD11b – Purified in vivo GOLD™ Functional Grade

Clone M1/70 is a monoclonal antibody widely used in in vivo mouse studies to target and detect CD11b (integrin ?M, Mac-1), a cell surface marker expressed on monocytes, macrophages, granulocytes, neutrophils, NK cells, dendritic cells, and some subsets of T and B cells.

Common uses in in vivo mouse studies include:

• Cell depletion: The M1/70 antibody can be administered to mice to selectively deplete CD11b? cells, such as monocytes, macrophages, and granulocytes, allowing researchers to assess the roles of these cells in various physiological or pathological conditions.
• Blocking function: In vivo administration of M1/70 can also block CD11b-mediated cell adhesion and complement receptor functions, including the binding of iC3b (a complement component) to Mac-1. This is useful for studying the importance of Mac-1 interactions in disease models.
• In vivo neutralization: The antibody is used to neutralize CD11b function during immune responses, inflammation, infection, or tissue injury. This allows for dissection of the role of CD11b in leukocyte trafficking, adhesion, phagocytosis, and other immune functions.
• Labeling and tracking: Although more commonly used ex vivo, M1/70 can be conjugated to fluorophores for in vivo imaging or cell tracking experiments, but this is less frequent compared to blocking and depletion applications.

Important technical notes:

• For in vivo work, low endotoxin and azide-free formulations (often labeled as “In Vivo Ready” or “Ultra-LEAF™”) are recommended to avoid toxicity or off-target effects.
• Dosing and timing vary by application and must be optimized for each experimental model.
• Controls (such as isotype controls and untreated animals) are essential for meaningful interpretation.

Summary Table: In Vivo Uses of M1/70 in Mice

References support robust use of clone M1/70 for in vivo depletion and blockade in mouse models of immunity, inflammation, and disease research.

M1/70 is a monoclonal antibody commonly used to detect CD11b (integrin ?M, Mac-1) in mice and humans, most frequently in flow cytometry, immunohistochemistry, and cell sorting. In the literature, it is frequently used alongside several other antibodies and markers to distinguish and characterize myeloid and leukocyte populations.

Commonly co-used antibodies or markers with M1/70 (anti-CD11b) include:

• CD45: A pan-leukocyte marker used to gate leukocytes and distinguish hematopoietic from non-hematopoietic cells.
• CD11c: Often used in combination with CD11b to separate dendritic cell subsets from other myeloid or monocyte cell types.
• F4/80: A hallmark marker for mouse macrophages, commonly used with CD11b to identify macrophage populations.
• Ly6G: A neutrophil-specific marker; co-staining with CD11b helps discriminate monocytes/macrophages from neutrophils.
• Gr-1 (Ly6C/Ly6G): Useful for distinguishing granulocyte and monocyte subsets, often in inflammatory or infection models.
• CD14: In human samples, CD14 is a classic monocyte marker. CD11b/CD14 dual-labeling is especially common in monocyte studies.
• CD3, CD19: T and B cell markers are often included in panels to exclude lymphocytes from myeloid gate strategies.

Additional proteins or antibodies may be selected depending on the specific experiment, tissue, and cell population of interest. For example:

• CD24: Used for further discrimination of myeloid subtypes (e.g., in TotalSeq panels).
• NK1.1: Used to assess or exclude NK cell populations.
• MHC-II (I-A/I-E): To assess activation or maturation states of antigen-presenting cells.

In summary, CD11b (M1/70) is typically used in combination with markers like CD45, CD11c, F4/80, Ly6G, Gr-1, CD14, CD24, NK1.1, and MHC-II to robustly identify, characterize, and sort myeloid and leukocyte subsets in immunology research. The specific combination depends on the experimental context and target cell populations.

Clone M1/70 is a rat monoclonal antibody widely used to identify and functionally characterize the CD11b integrin (Mac-1, ?M?2) on mouse myeloid cells. Key findings from scientific literature citing clone M1/70 include:

• Specificity and Applications:

  • M1/70 specifically binds to the 170?kDa ?M chain of CD11b in the Mac-1 integrin complex (CD11b/CD18), which is present on mouse granulocytes, monocytes/macrophages, dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes.
  • It is extensively used for flow cytometry, immunocytochemistry, and frozen immunohistochemistry to classify and quantify myeloid lineage cells.

• Functional Blocking:

  • The M1/70 antibody can block cell adherence and C3bi binding mediated by Mac-1, though it does not inhibit cell-mediated lysis.
  • Reports indicate cross-reactivity with human CD11b in some formats, expanding its utility.

• Inflammation and Immunomodulation:

  • M1/70 engages a strong pro-inflammatory response in Mac-1–expressing cells, notably macrophages, elevating cytokine production (e.g., IL-6, TNF-?, MCP-1) in vivo.
  • Use of M1/70 Fab fragments in mouse models allows analysis of leukocyte recruitment, rolling, and adhesion during inflammation, as observed by intravital microscopy.

• Leukocyte Recruitment:

  • Functional experiments with M1/70 demonstrate its ability to reduce myeloid cell infiltration in inflammatory and tumor microenvironments, suggesting a role in modulating immune cell trafficking.

• Tumor Response:

  • Neutralizing CD11b using monoclonal antibodies like M1/70 inhibits recruitment of myeloid cells into irradiated tumors, enhancing anti-tumor effects of radiotherapy.

• Technical Considerations:

  • For optimal results in flow cytometry and other assays, the manufacturer recommends titrating M1/70 and verifying isotype controls for proper interpretation.

In summary, clone M1/70 is foundational for the identification, quantification, and functional inhibition of CD11b+ cells in mouse models, especially in studies of myeloid biology, inflammation, and tumor immunology. It serves both as a cell marker and a functional blocking antibody, with a well-documented ability to modulate immune responses and leukocyte behavior.