Anti-Mouse CD11b – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD11b – Purified in vivo GOLD™ Functional Grade

Product No.: C377

[product_table name="All Top" skus="C377"]

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Clone
M1/70
Target
CD11b
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mac-1, Integrin αm chain, C3biR, CR3, Mo1, ITGAM
Isotype
Rat IgG2b κ
Applications
CyTOF®
,
FA
,
FC
,
ICC
,
IHC FF
,
in vivo
,
PhenoCycler®

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Select Product Size

Data

Anti-Mouse CD11b CyTOF™ Data
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
B10 mouse spleen cells enriched for T cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this M1/70 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
CyTOF®
CODEX®
ICC
IHC (Frozen)
Additional Reported Applications For Relevant Conjugates ?
B
Depletion
FA
FC
ICC
IHC (Frozen)
IHC (Paraffin)
IP
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone M1/70 recognizes the α subunit (CD11b) of the mouse CD11b/CD18 complex.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
Antigen Distribution
CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Function
Adhesion, chemotaxis
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity
.
Neuroscience
.
Neuroscience Cell Markers

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone M1/70 is commonly used in in vivo mouse studies to target CD11b-expressing cells, which include macrophages, monocytes, neutrophils, and some dendritic cells and NK cells. This antibody has several well-established functional applications in live animal research.

Depletion Studies

One of the primary in vivo applications is cell depletion, where M1/70 is used to selectively remove CD11b-expressing myeloid cells from the system. This approach allows researchers to study the functional role of these immune cell populations by observing the effects of their absence.

Blocking and Neutralization

M1/70 serves as a blocking antibody to inhibit CD11b function in vivo. The antibody can block iC3b binding to its receptor, thereby interfering with complement-mediated interactions and other CD11b-dependent cellular functions. This neutralization capacity makes it valuable for understanding the role of CD11b in various immune responses and inflammatory processes.

Imaging Applications

Recent applications include using radiolabeled M1/70 for PET-CT imaging to visualize bone marrow cell density changes in response to therapy. Specifically, 64Cu-labeled M1/70 has been employed to noninvasively assess functional bone marrow and monitor CD11b+ cell populations following chemotherapy treatments like Abraxane.

Detection and Phenotyping

Beyond functional manipulation, M1/70 is widely used as a marker antibody to detect and quantify CD11b expression on various myeloid cell populations in vivo, helping researchers track macrophages, granulocytes, neutrophils, and NK cells during experimental treatments.

For in vivo studies requiring high purity and low endotoxin levels, Ultra-LEAF™ purified formulations (with endotoxin < 0.01 EU/µg, azide-free, and 0.2 µm filtered) are specifically recommended to minimize unwanted immune activation.

Commonly used antibodies or proteins that are cited alongside M1/70 (anti-CD11b) in the literature for immunophenotyping or functional studies of myeloid and immune cell populations include:

  • F4/80 (BM8): Widely used as a murine macrophage marker, often in conjunction with CD11b to distinguish macrophage subsets.
  • CD68 (FA-11): Another marker for murine macrophages; used together with CD11b and F4/80 in multiparametric flow cytometry or immunohistochemistry.
  • CD18: Forms a heterodimer with CD11b (together called Mac-1 or CR3). Some studies use anti-CD18 antibodies to further resolve integrin subunits or defined functional receptor roles.
  • Ly6C, Ly6G: Common in myeloid panel combinations to distinguish between monocytes, neutrophils, and other granulocytes when co-stained with CD11b.
  • CD11c: Used to delineate dendritic cells (DCs), which can also express CD11b at varying levels.
  • CD45: A pan-leukocyte marker, typically included as part of lineage identification in immune cell profiling.
  • MHC class II (I-A/I-E, anti-MHC II): To define antigen-presenting cells, often paired with myeloid markers including CD11b.
  • CD14, CD16/32, CD115: For functional and subset identification of monocytes/macrophages.
  • PD-1, CTLA-4, and other checkpoint antibodies: Used in experimental contexts involving immunotherapy combinations, immune cell infiltration studies, or functional blockade studies, as seen in tumor microenvironment research.

These markers are frequently combined with M1/70 in multicolor flow cytometry panels, immunohistochemistry, and cell depletion or enrichment protocols to define, characterize, and manipulate mouse myeloid and related immune cell subsets.

Antibody/ProteinMajor Target/FunctionMain Use with M1/70
F4/80 (BM8)Murine macrophagesSubset discrimination
CD68 (FA-11)Murine macrophagesMacrophage identification
CD18β2 integrin subunit (Mac-1/CR3 formation)Functional/complex analysis
Ly6C/Ly6GMonocyte/neutrophil distinctionGranulocyte panels
CD11cDendritic cells & some myeloid cellsMyeloid/DC separation
CD45Pan-leukocyte markerLineage inclusion/exclusion
MHC class IIAntigen-presenting cells (DCs, macros)Functional assessment

These combinations allow researchers to resolve macrophage, monocyte, dendritic cell, and granulocyte populations with greater precision in mouse tissues, both at steady-state and in disease or experimental conditions.

Clone M1/70 is a widely used monoclonal antibody targeting CD11b (integrin alphaM), with key findings in the scientific literature highlighting its central role in immunology, tumor biology, and inflammation research.

Key findings include:

  • Broad utility in immunophenotyping and cell isolation:

    • M1/70 is used extensively in flow cytometry, immunoprecipitation, and immunohistochemistry (frozen tissue) to detect and quantify CD11b expression on murine myeloid cells, including macrophages, neutrophils, NK cells, and a subset of B cells.
    • It is also noted to be cross-reactive with human CD11b, useful for detecting this antigen on human monocytes, granulocytes, and NK cells.
  • Blocking/functional assays:

    • M1/70 has been critical in studies examining CD11b function, notably its ability to block ligand binding (such as iC3b and fibrinogen) to the Mac-1 integrin, and to inhibit myeloid cell recruitment and adhesion in vitro and in vivo.
    • Functional grade M1/70 is used for assays requiring neutralization or inhibition of CD11b-mediated cellular functions, such as leukocyte migration and cell-cell adhesion.
  • Role in tumor biology and angiogenesis:

    • Studies using M1/70 demonstrate that neutralizing CD11b reduces myeloid cell infiltration into irradiated tumors and enhances tumor response to radiotherapy, illustrating the importance of CD11b^+^ myeloid cells in tumor vasculature restoration and recurrence after irradiation.
    • This work highlights the functional relevance of CD11b in tumor microenvironment modulation and post-irradiation angiogenesis.
  • Inflammation and cytokine response:

    • M1/70 injection can induce a pro-inflammatory response in Mac-1–expressing cells, especially in macrophages, leading to increased serum cytokines like IL-6, TNF-α, and MCP-1.
  • Antibody characteristics and limitations:

    • M1/70 is not recommended for immunohistochemistry on formalin-fixed paraffin-embedded tissues, but is validated for immunofluorescence on frozen tissues and for in vitro and in vivo blocking studies.
    • Purity and performance characteristics have been validated: >90% by SDS-PAGE, <10% aggregation by HPLC, and tested for low endotoxin when used in cell culture or in vivo.

Summary Table: Main Applications and Key Insights

ApplicationKey Use/DiscoveryLimitations/Notes
Flow cytometryMyeloid cell profiling in mouse/human tissueNot compatible with fixed, paraffin-embedded IHC
ImmunoprecipitationProtein-protein interaction studiesUse for cell lysate studies
ImmunohistochemistryFrozen tissue onlyNot for formalin-fixed tissue
Functional blockingInhibits CD11b ligand binding and functionFunctional grade antibody required
Cancer researchBlocks myeloid recruitment/angiogenesisReveals role in tumor responses to irradiation
Inflammation studiesInduces cytokine response in vivoConsider in vivo immune effects

In summary, clone M1/70 is indispensable for dissecting myeloid cell biology, integrin function, inflammatory processes, and the tumor microenvironment. Its blocking ability allows not just for cell marking, but also for probing the functional consequences of CD11b inhibition in experimental systems.

References & Citations

1.) Springer, T.A., et al. (1978) Eur. J. Immunol. 8:539
2.) Springer, T.A., et al. (1982) Immunol. Rev. 68:171
3.) Ding, A., et al. (1987) J Exp Med. 165:733
4.) Vignali, D. A. et al. (1990) J. Immunol. 144:4030
5.) Kantor, A. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:3320
6.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030 Journal Link
CyTOF®
FA
Flow Cytometry
ICC
IHC FF
in vivo Protocol
PhenoCycler®

Certificate of Analysis

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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.