Anti-Mouse CD11b – Purified in vivo PLATINUM™ Functional Grade

Clone M1/70 is an anti-mouse (and anti-human) CD11b monoclonal antibody commonly used in in vivo mouse studies to detect, deplete, block, or neutralize CD11b-expressing cells such as macrophages, monocytes, neutrophils, and some dendritic cells and NK cells.

Key in vivo applications of M1/70 in mice include:

• Cell Depletion: M1/70 can be administered to live mice to deplete CD11b+ cells (mainly macrophages, monocytes, and neutrophils) to study the function of these cells in immune responses, inflammation, or disease models.
• Blocking/Neutralization: The antibody is widely used to block CD11b-mediated adhesion and signaling, thereby inhibiting leukocyte recruitment, migration, or function in various models (e.g., inflammation, cancer, infection).
• Flow Cytometric Tracking: In some cases, M1/70 can be used to label CD11b+ cells in vivo (with appropriate fluorophore-conjugation), allowing their tracking or quantification after tissue collection.

Best practices:
For in vivo use, M1/70 must be in a highly purified, endotoxin-free, and azide-free formulation to minimize any adverse reactions; products such as Ultra-LEAF™ purified antibody or InVivoMAb formulations are specifically recommended for live animal studies.

Antibody properties and alternatives:

• Isotype: Rat IgG2b
• Other applications: Immunohistochemistry, immunocytochemistry, immunofluorescence, immunoprecipitation, cell sorting.
• M1/70 is also reported to block iC3b binding to its receptor on myeloid cells, highlighting its utility in studies involving complement pathways.

Summary of common experimental uses:

Note: While M1/70 is suitable for many in vivo applications, always ensure the chosen product is specifically formulated for animal injection (ultra-low endotoxin, azide-free, sterile).

If you need protocols or details for a specific mouse model or experimental design, please specify.

The correct storage temperature for sterile packaged clone M1/70 depends on whether your use will be short-term or long-term:

• For short-term storage (up to two weeks): Store at 4°C (refrigerator temperature).
• For long-term storage: Aliquot and store at -20°C or -80°C (freezer or ultra-low freezer); avoid repeated freeze-thaw cycles for optimal antibody integrity.

Storing at these temperatures ensures the stability and effectiveness of the antibody. For best results, aliquot into volumes of at least 20 µl and, if recommended for your product format, add an equal volume of glycerol as a cryoprotectant before freezing.

Commonly used antibodies or proteins with M1/70 (anti-CD11b) in the literature include markers for distinguishing myeloid cells, monocytes, macrophages, dendritic cells, T cells, B cells, and for cell subset identification in immunology and neuroscience research.

Key antibodies and proteins often used with M1/70:

• CD45: Universal leukocyte marker for gating immune cell populations.
• CD11c: Dendritic cell marker, used to distinguish between macrophages (CD11b+) and dendritic cells (CD11c+).
• F4/80: Macrophage-specific marker for mouse tissues.
• Ly6G: Granulocyte/neutrophil marker, often to exclude neutrophils (CD11b+Ly6G+).
• CD14: Monocyte marker, especially in human studies.
• CD24: Used for distinguishing cell subsets in flow cytometry.

Other commonly reported markers:

• CD3: T cell marker.
• CD19 or B220: B cell marker, helps exclude B cells (CD11b?B220+).
• MHC-II: For antigen-presenting cell identification.
• NK1.1 or CD49b: Natural Killer (NK) cell markers.

Researchers select combinations like CD11b (M1/70), CD45, CD11c, F4/80, Ly6G, and lineage-specific markers according to the cell types and experimental objectives, especially in multiparametric flow cytometry, immunohistochemistry, or spatial biology studies.

For neuroscience, Iba1 is also used with CD11b to mark microglia specifically.

CD11b is itself detected with M1/70 in mouse and human systems, making it a standard for myeloid cell identification in primary tissues and sorted populations.

Many studies referenced in product literature report panels utilizing the above markers for precise immune cell subset gating and microenvironment analysis.

Clone M1/70 is a widely utilized rat monoclonal antibody targeting mouse CD11b (?M integrin, Mac-1/CD11b/CD18), and its key findings in scientific literature include:

• Specificity and Mechanism: M1/70 binds to the 170 kDa ?M integrin chain on mouse CD11b, a component of the Mac-1 integrin complex (CD11b/CD18), which is important in leukocyte adhesion, migration, and immune response regulation.
• Cell Type Detection: It is commonly used in flow cytometry and immunochemical applications to identify myeloid cells, including granulocytes, monocytes/macrophages, dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. It facilitates discrimination between myeloid and non-myeloid cells due to differential CD11b expression.
• Functional Insights:
  • M1/70 can block cell adherence and C3bi binding but does not block cell-mediated lysis, indicating selective interference with Mac-1 functional pathways.
  • It reportedly induces a pro-inflammatory response in Mac-1–expressing cells (particularly macrophages) and elevates cytokine levels such as IL-6, TNF?, and MCP-1 when injected in vivo, suggesting involvement in inflammation signaling processes.
  • The antibody has been employed to study leukocyte recruitment and migration during inflammatory responses, showing that it affects adhesion and rolling of leukocytes on endothelial surfaces, as well as modulating cytokine profiles after in vivo administration.
  • Notably, M1/70 F(ab) fragments have been shown not to impact venous thrombosis or platelet effector function, indicating a degree of selectivity in its immune-modulating effects.
• Tumor Immunology Applications:
  • Neutralization of CD11b with M1/70 reduces radiation-induced infiltration of myeloid cells into tumors, supporting its use in research on immune cell recruitment to cancer microenvironments.
• Experimental Guidance:
  • The antibody is validated for multiple applications including flow cytometry, immunohistochemistry, and immunocytochemistry.
  • Application-specific titration and use of isotype controls are recommended for optimal experimental outcomes.

In summary, clone M1/70 is instrumental for identifying, isolating, and modulating myeloid cells in immunological studies, helping elucidate leukocyte migration, inflammation, and immune responses, while also serving as a tool to investigate the role of CD11b in cancer and other disease models.