Anti-Mouse CD172a – DyLight® 650

Anti-Mouse CD172a – DyLight® 650

Product No.: P392

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
IgG1 κ

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Anti-Mouse CD172a CyTOF™ Data
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Antibody Details

Product Details

Reactive Species
Host Species
Mouse brain membrane protein
Product Concentration
0.2 mg/ml
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day 2-8°C
Excitation Laser
Red Laser (652 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this P84 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Clone P84 recognizes an epitope on mouse CD172a.
CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)1. SIRPα is expressed predominantly in myeloid cells2 - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells3. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])4, is expressed in most cell types5. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells6,7. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages11. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics12.
Antigen Distribution
CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells.

Antigen Details

CD47, SP-A, SP-D
Negative regulation of several biological processes
NCBI Gene Bank ID
Research Area
Cell Adhesion
Innate Immunity

References & Citations

1. Fujioka, Y., et al. (1996) Mol. Cell. Biol. 16:6887
2. Adams, S., et al. (1998) J. Immunol. 161:1853
3. Chuang W, et al. (1990) Dev Biol. 137:219–232
4. Seiffert, M., et al. (1999) Blood 94:3633
5. Oldenborg P. A. (2013) ISRN Hematol. 2013:614619
6. Oldenborg, P. A., et al. (2000) Science 288:2051
7. Oldenborg, P. A., et al. (2001) J. Exp. Med. 193:855
8. Brooke, G. P., et al. (1998) Eur. J. Immunol. 28:1
9. Seiffert, M., et al. (2001) Blood 97:2741
10. Latour, S., et al. (2001) J. Immunol. 167:2547
11. Jaiswal S, et al. (2009) Cell. 138(2):271-85
12. Jalil AR, et al. (2020) Antib Ther. 3(2):80-94
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.