Anti-Mouse CD18 (Clone C71/16) – Purified in vivo GOLD™ Functional Grade

The C71/16 clone is a rat monoclonal antibody against mouse CD18 (integrin ?2), and in in vivo mouse studies, it is primarily used for detecting and functionally blocking the CD18 molecule on leukocytes.

Key uses based on published studies and product information include:

• Phenotypic Analysis (Flow Cytometry):

  • C71/16 is commonly used to stain mouse leukocytes (e.g., splenocytes, peritoneal macrophages) to assess CD18 expression by flow cytometry.
  • In such experiments, tissues or isolated cells from mice (such as splenocytes or peritoneal macrophages) are stained ex vivo after collection, allowing quantification or gating of CD18+ populations.

• Functional Blocking Antibody In Vivo:

  • C71/16 can be used to functionally block CD18 integrin activity in mice. Studies have treated mice or isolated mouse immune cells with this antibody to transiently inhibit CD18-dependent adhesion and trafficking of leukocytes, which is relevant in inflammation, infection, and immunological models.
  • In mouse studies examining migration, adhesion, or involvement of ?2 integrins in disease (such as in models of arthritis, atherosclerosis, or infection), the antibody is administered systemically (e.g., intravenously or intraperitoneally) to investigate the effect of CD18 blockade on disease development or immune response.
  • Example application: C71/16 was used to block ?2 integrin function on peritoneal macrophages isolated from mice, modulating their interaction with pathogens in vitro and ex vivo.

• Experimental Designs in Published Work:

  • One example study harvested peritoneal macrophages from thioglycolate-inflamed mice, treated them with C71/16 to block CD18, and analyzed functional outcomes such as pathogen binding or migration.
  • Another application includes using C71/16 in multiple antibody panels for integrin phenotyping in tumor/immune response models.

In summary: Clone C71/16 is used in mice to stain or block the CD18 integrin. In vivo applications often involve systemic administration to block leukocyte adhesive functions or ex vivo staining of mouse tissues/cells for immunophenotyping. The specific dose, route, and timing may vary by study and experimental question.

The correct storage temperature for a sterile packaged clone, such as C71/16, largely depends on the nature of the biological material. Here are some general guidelines for the storage of biological samples:

1. Refrigerated Storage: For samples that require enzymatic activity or are sensitive to freezing, temperatures between 2°C and 8°C (36°F to 46°F) are often used. This is common for enzymes, antibodies, and certain biological reagents.

2. Deep Freezer Storage: For many biological samples such as DNA, RNA, proteins, and other molecules that require long-term storage without degradation, temperatures between -20°C and -40°C are commonly used. However, ultra-low freezers with temperatures ranging from -70°C to -93°C are preferred for long-term preservation of sensitive biological materials.

3. Controlled Room Temperature (CRT): If the clone is stable at room temperature and includes preservatives, a temperature range of 20°C to 25°C (68°F to 77°F) can be used. This is typically for materials that should not be frozen to avoid degradation.

Without specific details on the nature of clone C71/16, it is difficult to provide a precise storage temperature. However, for most biological or genetic materials, a deep freezer or ultra-low freezer is commonly used for long-term preservation. If you have more specific information about the clone, such as its composition or the presence of preservatives, you can tailor the storage conditions accordingly.

Other commonly used antibodies or proteins studied alongside C71/16 (which targets mouse CD18 or Integrin ?2) are typically those that recognize molecules associated with the various CD11/CD18 integrin heterodimers—specifically, antibodies against CD11a (LFA-1, ?L?2 integrin), CD11b (Mac-1, ?M?2 integrin), and CD11c (?X?2 integrin).

Supporting details:

• CD18 (targeted by C71/16) is the ?2 subunit common to several integrins; as such, it associates non-covalently with CD11a, CD11b, and CD11c to form LFA-1, Mac-1, and p150,95, respectively.
• In studies regarding immune cell adhesion, trafficking, and activation, antibodies against the respective ?-chains (CD11a, CD11b, CD11c) are frequently used together with anti-CD18 to distinguish and characterize specific cell subsets and their functional properties.
• Common applications for these antibody combinations include flow cytometry, immunohistochemistry, immunocytochemistry, immunoprecipitation, Western blot, and ELISA, as referenced by product documentation and literature.

Frequently co-used markers/proteins:

• CD11a: Integrin ?L, forming LFA-1 with CD18; marks T cells and other leukocytes.
• CD11b: Integrin ?M, forming Mac-1 with CD18; prevalent on monocytes, neutrophils, and macrophages.
• CD11c: Integrin ?X, forming p150,95 with CD18; identifies dendritic cells and certain monocyte subsets.

Additional context:

• Isotype controls, such as Rat IgG2a antibodies, are often used alongside C71/16 in multi-color staining protocols to verify specificity.
• Other cell-type and activation markers (e.g., CD45, CD3, CD4) may be included in broader panels, but the most direct biochemical partners for CD18 in immunological research remain the CD11 family.

Summary Table:

This panel is foundational for dissecting leukocyte population structure and function, especially in research focusing on adhesion and migration.

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