Anti-Mouse CD18 (Clone C71/16) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD18 (Clone C71/16) – Purified in vivo GOLD™ Functional Grade
Product No.: C378
Clone C71/16 Target CD18 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Integrin beta-2, Mac-1 beta, macrophage antigen-1 beta, complement receptor C3 subunit beta, lymphocyte function associated antigen 1, cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, 2E6, LAD, Cd18, Lfa1, MF17, LCAMB, AI528527 Isotype Rat IgG2a Applications FA , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Cell membrane glycoproteins from mouse T-cell lymphoma BW5147 Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737481 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this C71/16 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this C71/16 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone C71/16 recognizes an epitope on mouse CD18. Background CD18 is a 95kD type I transmembrane glycoprotein. CD18 is the Integrin β2 chain and forms multiple heterodimers with the three integrin α chains, CD11a, CD11b, and CD11c. CD18 binds with CD11a to form LFA-1. LFA-1 is involved in adhesion and binding to antigen presenting cells via interactions with ICAM-1. CD18 binds with CD11b and CD11c forming complement receptors , such as MAC-1, that participate in the innate immune response by recognizing foreign antigen peptides and destroying them via phagocytosis. In humans, lack of functional CD18 causes Leukocyte Adhesion Deficiency (LAD) which is characterized by the inability of circulating leukocytes to respond to foreign bodies, reducing the ability of the immune system to fight off infection. Antigen Distribution The mouse CD18 antigen is present on mouse T-cells, B-cells, NK cells, monocytes, macrophages and granulocytes. Ligand/Receptor ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, fibrinogen PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Neuroinflammation . Neuroscience Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone C71/16 is a rat monoclonal antibody that binds specifically to mouse CD18 (integrin β2, the common β subunit of LFA-1 and Mac-1), and its most common in vivo applications in mice are in studies of immune cell adhesion, trafficking, and function, particularly through functional blocking and detection of CD18 on leukocytes. Core in vivo applications include:
Supporting context:
Summary table:
If you require specific in vivo disease models or protocols where C71/16 is employed, provide the context (e.g., autoimmune, infectious, or transplant models), as application details can vary by field. The C71/16 monoclonal antibody specifically targets mouse CD18, which is a common β2 integrin subunit crucial in forming various integrin heterodimers like CD11a/CD18 (αLβ2 integrin) . While the search results do not specifically mention other antibodies or proteins commonly used with C71/16, researchers often employ a combination of antibodies to study complex immunological processes. Commonly used antibodies in immunological research that might be used in conjunction with C71/16 include:
In terms of proteins, molecules involved in cell adhesion and signaling, such as ICAM-1 and VCAM-1, are studied in conjunction with CD18 as they are ligands for some of the integrin complexes formed with CD18. These combinations allow researchers to explore the complex interactions and functions within the immune system effectively. Key findings from scientific literature citing clone C71/16 predominantly concern its use as a monoclonal antibody for rat CD18 detection, facilitating research on leukocyte integrins, cell adhesion, and immune responses.
Additional context:
In summary, clone C71/16 is recognized in scientific literature as a critical reagent for functional and phenotypic analysis of rat β2 integrins, thereby enabling discoveries in leukocyte biology, inflammation, and immune regulation. No dosing regimens for the antibody clone C71/16 are reported in the provided search results. The sources include detailed dosing protocols for other antibody clones (such as anti-PD-1 or anti-CTLA-4), as well as general discussions of antibody dosages in mouse models, but there is no mention of C71/16 specifically. Essential context:
Recommendations if you are optimizing C71/16 dosing:
If you need C71/16-specific data:
In summary, without specific published data for C71/16, only general guidance based on similar antibody regimens in mouse models can be offered, and C71/16-specific studies should be consulted directly for precise regimens. References & CitationsSpringer, T. et al. (1978) Eur. J. Immunol. 8:539 Technical ProtocolsCertificate of Analysis |
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