Anti-Mouse CD25 (Clone PC61) – Alexa Fluor® 488
Anti-Mouse CD25 (Clone PC61) – Alexa Fluor® 488
Product No.: C1916AF
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Clone PC61 Target CD25 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names IL-2Rα, Ly-43, p55, Tac Isotype Rat IgG1 λ Applications FC |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1 Product Concentration 0.2 mg/ml Formulation This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Alexa Fluor® is a trademark of Life Technologies Corporation. State of Matter Liquid Storage and Handling This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Excitation Laser Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? B Depletion IHC (Frozen) IP For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone PC61 recognizes an epitope on mouse CD25. Background CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25. Antigen Distribution CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. Ligand/Receptor IL-2 PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051 2. FW Alt et al. (1995) Immnnity 3: 521 3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S Technical Protocols Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C1924 | |
C1189 | |
C1193 | |
C1192 | |
C1191 | |
C1916 | |
C1917 | |
C1918 | |
C1922 | |
C1920 | |
C526 | |
C1194 | |
C1190 | |
C1916AF | |
C2845 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
